Research workbench
Maltose-bound maltose sensor engineered by insertion of circularly permuted green fluorescent protein into E. COLI maltose binding protein at position 311
Analyse Maltose-bound maltose sensor engineered by insertion of circularly permuted… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3OSR
- Type
- protein
- Length
- 1,203 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Maltose-binding periplasmic PROTEIN,GREEN fluorescent protein
- UniProt
- P0AEY0, P42212
- Ligands
- C12, GLC
- Pockets
- 11 · best druggability 0.77
Structure
x-ray diffraction · 2 distinct chains · 32% helix, 32% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Maltose-binding periplasmic PROTEIN,GREEN fluorescent protein | protein | 603 | 33% helix, 32% strand | P0AEY0, P42212 |
| B | Maltose-binding periplasmic PROTEIN,GREEN fluorescent protein | protein | 600 | 32% helix, 32% strand | P0AEY0, P42212 |
Ligands and binding sites
- C12 · 2-(1-amino-2-hydroxypropyl)-4-(4-hydroxybenzyl)-1-(2-oxoethyl)-1H-imidazol-5-olate · ligand · 2 copies · chains A, B
- GLC · alpha-D-glucopyranose · sugar · 4 copies · chains A, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
11 pockets found on a 1 Å grid, 8 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 127 | 0.77 | 0.95 | 28 | C12 |
| 2 | 318 | 0.75 | 0.87 | 36 | empty |
| 3 | 144 | 0.75 | 0.93 | 29 | C12 |
| 4 | 402 | 0.74 | 0.84 | 45 | empty |
| 5 | 494 | 0.70 | 0.80 | 63 | empty |
Interfaces
1 interface between chains.
- A–B · 957 Ų buried · 14 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 4 covalent (covalent ligand).
Compared structures
- 3OSR · Maltose-bound maltose sensor engineered by insertion of circularly permuted green… · the same entry in another collection · chain A against A, TM-score 1.00
- P42212 · AlphaFold monomer V2.0 prediction for green fluorescent protein (P42212) · a predicted model of the same protein · chain B against A, TM-score 0.24
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P0AEY0
- Every structure of UniProt P42212
- Elsewhere: RCSB PDB 3OSR · PDBe 3OSR · UniProt P0AEY0 · UniProt P42212
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