Research workbench
Structures of actin-bound WH2 domains of spire and the implication for filament nucleation
Analyse Structures of actin-bound WH2 domains of spire and the implication for filament… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3MN5
- Type
- protein
- Length
- 367 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Actin, alpha skeletal muscle; Protein spire
- UniProt
- P68135, Q9U1K1
- Ligands
- ATP, LAB, CA
- Pockets
- 2 · best druggability 0.73
Structure
x-ray diffraction · 2 distinct chains · 45% helix, 19% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Actin, alpha skeletal muscle | protein | 347 | 44% helix, 20% strand | P68135 |
| S | Protein spire | protein | 20 | 55% helix, 5% strand | Q9U1K1 |
Ligands and binding sites
- ATP · Adenosine-5'-triphosphate · ligand · chains A
- LAB · Latrunculin B · ligand · chains A
- CA · Calcium ion · metal · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
2 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 321 | 0.73 | 0.86 | 46 | CA, ATP, LAB |
| 2 | 182 | 0.55 | 0.79 | 28 | empty |
Interfaces
1 interface between chains.
- A–S · 865 Ų buried · 11 hydrogen bonds · protein-protein
Compared structures
- 3MN5 · Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation · the same entry in another collection · chain A against A, TM-score 1.00
- P68135 · AlphaFold monomer V2.0 prediction for actin, alpha skeletal muscle (P68135) · a predicted model of the same protein · chain A against A, TM-score 0.99
- Q9U1K1 · AlphaFold monomer V2.0 prediction for protein spire (Q9U1K1) · a predicted model of the same protein · chain S against A, TM-score 0.50
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P68135
- Every structure of UniProt Q9U1K1
- Elsewhere: RCSB PDB 3MN5 · PDBe 3MN5 · UniProt P68135 · UniProt Q9U1K1
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.