Crystal structure of human 14-3-3 sigma in complex with RAF1 peptide (6MER) and stabilisator fusicoccin

Structure

x-ray diffraction · 2 distinct chains · 78% helix, 0% strand (DSSP).

Ligands and binding sites

• FSC · Fusicoccin · ligand · chains A, P
• MG · Magnesium ion · metal · 4 copies · chains A
• CL · Chloride ion · ion · 2 copies · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

2 pockets found on a 1 Å grid, 0 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–P · 536 Ų buried · 12 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 2 modified (oxidation, phosphorylation).

Compared structures

• 3IQV · Crystal Structure of human 14-3-3 sigma in Complex with Raf1 peptide (6mer) and… · the same entry in another collection · chain A against A, TM-score 1.00
• P31947 · AlphaFold monomer V2.0 prediction for 14-3-3 protein sigma (P31947) · a predicted model of the same protein · chain A against A, TM-score 0.95
• P04049 · AlphaFold monomer V2.0 prediction for RAF proto-oncogene SERINE/THREONINE-protein kinase… · a predicted model of the same protein · chain P against A, TM-score 0.62

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P31947
• Every structure of UniProt P04049
• Elsewhere: RCSB PDB 3IQV · PDBe 3IQV · UniProt P31947 · UniProt P04049