Research workbench
Impaired binding of 14-3-3 to RAF1 is linked to noonan and leopard syndrome
Analyse Impaired binding of 14-3-3 to RAF1 is linked to noonan and leopard syndrome in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3CU8
- Type
- protein
- Length
- 474 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- 14-3-3 protein ZETA/DELTA; RAF proto-oncogene SERINE/THREONINE-protein kinase
- EC
- 2.7.11.1
- UniProt
- P63104, P04049
- Ligands
- MG
- Pockets
- 5 · best druggability 0.69
Structure
x-ray diffraction · 4 distinct chains · 78% helix, 0% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | 14-3-3 protein ZETA/DELTA | protein | 229 | 82% helix, 0% strand | P63104 |
| B | 14-3-3 protein ZETA/DELTA | protein | 228 | 80% helix, 0% strand | P63104 |
| P | RAF proto-oncogene SERINE/THREONINE-protein kinase (EC 2.7.11.1) | protein | 9 | 0% helix, 0% strand | P04049 |
| Q | RAF proto-oncogene SERINE/THREONINE-protein kinase (EC 2.7.11.1) | protein | 8 | 0% helix, 0% strand | P04049 |
Ligands and binding sites
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
5 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 180 | 0.69 | 0.87 | 30 | empty |
| 2 | 170 | 0.67 | 0.86 | 29 | empty |
| 3 | 284 | 0.50 | 0.74 | 25 | empty |
| 4 | 55 | 0.34 | 0.77 | 13 | empty |
| 5 | 50 | 0.33 | 0.78 | 15 | empty |
Interfaces
3 interfaces between chains.
- A–B · 973 Ų buried · 13 hydrogen bonds · protein-protein
- A–P · 746 Ų buried · 18 hydrogen bonds · protein-protein
- B–Q · 665 Ų buried · 15 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 2 modified (phosphorylation).
Compared structures
- 3CU8 · Impaired binding of 14-3-3 to Raf1 is linked to Noonan and LEOPARD syndrome · the same entry in another collection · chain Q against Q, TM-score 1.00
- P63104 · AlphaFold monomer V2.0 prediction for 14-3-3 protein ZETA/DELTA (P63104) · a predicted model of the same protein · chain A against A, TM-score 0.99
- P04049 · AlphaFold monomer V2.0 prediction for RAF proto-oncogene SERINE/THREONINE-protein kinase… · a predicted model of the same protein · chain Q against A, TM-score 0.66
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P63104
- Every structure of UniProt P04049
- Elsewhere: RCSB PDB 3CU8 · PDBe 3CU8 · UniProt P63104 · UniProt P04049
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