Research workbench

Isometrically contracting insect asynchronous flight muscle

Analyse Isometrically contracting insect asynchronous flight muscle in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 2W49
Type: protein
Length: 8,776 residues
Chains: 36
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Troponin C, skeletal muscle; Troponin T, fast skeletal muscle isoforms; Troponin I, fast…
UniProt: P02588, P12620, P68246, P58772, P68135
Ligands: CA
Pockets: 20 · best druggability 0.80

Structure

electron microscopy · 6 distinct chains · 68% helix, 7% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
0, 3, 6, 9	Troponin C, skeletal muscle	protein	159	63% helix, 3% strand	P02588
1, 4, 7, Y	Troponin T, fast skeletal muscle isoforms	protein	90	77% helix, 0% strand	P12620
2, 5, 8, Z	Troponin I, fast skeletal muscle	protein	141	65% helix, 0% strand	P68246
A, B, U, V	Tropomyosin alpha-1 chain	protein	277	99% helix, 0% strand	P58772
C, T, W, X	Tropomyosin alpha-1 chain	protein	39	95% helix, 0% strand	P58772
D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S	Actin, alpha skeletal muscle	protein	372	42% helix, 20% strand	P68135

Ligands and binding sites

CA · Calcium ion · metal · 16 copies · chains 0, Y, 3, 1, 6, 4, 9, 7

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	1073	0.80	0.81	108	empty
2	931	0.79	0.81	94	empty
3	1137	0.78	0.80	102	empty
4	939	0.78	0.80	99	empty
5	1006	0.78	0.80	97	empty

Interfaces

92 interfaces between chains.

U–V · 7378 Å² buried · 47 hydrogen bonds · protein-protein
A–B · 7372 Å² buried · 47 hydrogen bonds · protein-protein
Y–Z · 2430 Å² buried · 21 hydrogen bonds · protein-protein
7–8 · 2420 Å² buried · 21 hydrogen bonds · protein-protein
1–2 · 2419 Å² buried · 21 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 1 isopeptide (isopeptide).

Compared structures

2W49 · Isometrically contracting insect asynchronous flight muscle · the same entry in another collection · chain 6 against 6, TM-score 1.00

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P02588
Every structure of UniProt P12620
Every structure of UniProt P68246
Every structure of UniProt P58772
Every structure of UniProt P68135
Elsewhere: RCSB PDB 2W49 · PDBe 2W49 · UniProt P02588 · UniProt P12620 · UniProt P68246 · UniProt P58772 · UniProt P68135

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