Research workbench
Crystal structure of the regulatory fragment of mammalian ampk in complexes with ATP-AMP
Analyse Crystal structure of the regulatory fragment of mammalian ampk in complexes… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 2V92
- Type
- protein
- Length
- 479 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein…
- EC
- 2.7.11.1
- UniProt
- P54645, O43741, P80385
- Ligands
- ATP, AMP
- Pockets
- 8 · best druggability 0.79
Structure
x-ray diffraction · 3 distinct chains · 32% helix, 28% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | 5'-AMP-activated protein kinase catalytic subunit alpha-1 (EC 2.7.11.1) | protein | 102 | 27% helix, 37% strand | P54645 |
| B | 5'-AMP-activated protein kinase subunit beta-2 | protein | 73 | 0% helix, 38% strand | O43741 |
| E | 5'-AMP-activated protein kinase subunit gamma-1 | protein | 304 | 41% helix, 22% strand | P80385 |
Ligands and binding sites
- ATP · Adenosine-5'-triphosphate · ligand · 2 copies · chains E
- AMP · Adenosine monophosphate · ligand · chains E
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
8 pockets found on a 1 Å grid, 5 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1027 | 0.79 | 0.80 | 94 | ATP, AMP |
| 2 | 53 | 0.60 | 0.91 | 16 | empty |
| 3 | 210 | 0.55 | 0.79 | 25 | empty |
| 4 | 186 | 0.54 | 0.79 | 42 | empty |
| 5 | 206 | 0.51 | 0.76 | 34 | empty |
Interfaces
3 interfaces between chains.
- A–B · 1658 Ų buried · 22 hydrogen bonds · protein-protein
- B–E · 966 Ų buried · 15 hydrogen bonds · protein-protein
- A–E · 711 Ų buried · 17 hydrogen bonds · protein-protein
Compared structures
- 2V92 · Crystal structure of the regulatory fragment of mammalian AMPK in complexes with ATP-AMP · the same entry in another collection · chain A against A, TM-score 1.00
- P80385 · AlphaFold monomer V2.0 prediction for 5'-AMP-activated protein kinase subunit gamma-1… · a predicted model of the same protein · chain E against A, TM-score 0.96
- P54645 · AlphaFold monomer V2.0 prediction for 5'-AMP-activated protein kinase catalytic subunit… · a predicted model of the same protein · chain A against A, TM-score 0.90
- O43741 · AlphaFold monomer V2.0 prediction for 5'-AMP-activated protein kinase subunit beta-2… · a predicted model of the same protein · chain B against A, TM-score 0.69
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P54645
- Every structure of UniProt O43741
- Every structure of UniProt P80385
- Elsewhere: RCSB PDB 2V92 · PDBe 2V92 · UniProt P54645 · UniProt O43741 · UniProt P80385
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