0.9A resolution crystal structure of alpha-lytic protease complexed with a transition state analogue, meosuc-ala-ala-pro-val boronic acid

Structure

x-ray diffraction · 2 distinct chains · 7% helix, 55% strand (DSSP).

Ligands and binding sites

• MSU · Succinic acid monomethyl ester · ligand · chains A, B
• B2V · Valine boronic acid · ligand · chains A, B
• SO4 · Sulfate ion · ion · 8 copies · chains A, B
• GOL · Glycerol · additive · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

1 interface between chains.

• A–B · 229 Ų buried · 3 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 3 covalent, 3 disulfide (covalent ligand, disulfide).

Compared structures

• 2H5D · 0.9A resolution crystal structure of alpha-lytic protease complexed with a transition… · the same entry in another collection · chain A against A, TM-score 1.00
• P00778 · AlphaFold monomer V2.0 prediction for alpha-lytic protease (P00778) · a predicted model of the same protein · chain A against A, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P00778
• Elsewhere: RCSB PDB 2H5D · PDBe 2H5D · UniProt P00778