Research workbench
Structure of E. COLI RNA polymerase beta' G/G' insert
Analyse Structure of E. COLI RNA polymerase beta' G/G' insert in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 2AUK
- Type
- protein
- Length
- 924 residues
- Chains
- 5
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- DNA-directed RNA polymerase beta' chain
- EC
- 2.7.7.6
- UniProt
- P0A8T7
- Pockets
- 11 · best druggability 0.81
Structure
x-ray diffraction · 4 distinct chains · 4% helix, 42% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | DNA-directed RNA polymerase beta' chain (EC 2.7.7.6) | protein | 189 | 2% helix, 41% strand | P0A8T7 |
| B | DNA-directed RNA polymerase beta' chain (EC 2.7.7.6) | protein | 181 | 4% helix, 43% strand | P0A8T7 |
| C, D | DNA-directed RNA polymerase beta' chain (EC 2.7.7.6) | protein | 183 | 4% helix, 43% strand | P0A8T7 |
| E | DNA-directed RNA polymerase beta' chain (EC 2.7.7.6) | protein | 188 | 4% helix, 41% strand | P0A8T7 |
Pockets
11 pockets found on a 1 Å grid, 7 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1194 | 0.81 | 0.82 | 143 | empty |
| 2 | 462 | 0.72 | 0.82 | 65 | empty |
| 3 | 380 | 0.70 | 0.82 | 44 | empty |
| 4 | 217 | 0.58 | 0.80 | 47 | empty |
| 5 | 279 | 0.57 | 0.77 | 44 | empty |
Interfaces
4 interfaces between chains.
- A–D · 1354 Ų buried · 15 hydrogen bonds · protein-protein
- D–E · 1302 Ų buried · 14 hydrogen bonds · protein-protein
- B–C · 1281 Ų buried · 17 hydrogen bonds · protein-protein
- A–B · 1197 Ų buried · 14 hydrogen bonds · protein-protein
Compared structures
- 2AUK · Structure of E. coli RNA polymerase beta' G/G' insert · the same entry in another collection · chain E against E, TM-score 1.00
- P0A8T7 · AlphaFold monomer V2.0 prediction for DNA-directed RNA polymerase subunit beta' (P0A8T7) · a predicted model of the same protein · chain C against A, TM-score 0.92
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P0A8T7
- Elsewhere: RCSB PDB 2AUK · PDBe 2AUK · UniProt P0A8T7
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