Research workbench
Alternative splicing of RAC1 generates RAC1B, a self-activating GTPase
Analyse Alternative splicing of RAC1 generates RAC1B, a self-activating GTPase in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 1RYF
- Type
- protein
- Length
- 337 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- RAS-related C3 botulinum toxin substrate 1 isoform RAC1B
- EC
- 3.6.5.2
- UniProt
- P63000
- Ligands
- GDP, MG
- Pockets
- 4 · best druggability 0.59
Structure
x-ray diffraction · 2 distinct chains · 38% helix, 27% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | RAS-related C3 botulinum toxin substrate 1 isoform RAC1B (EC 3.6.5.2) | protein | 170 | 38% helix, 26% strand | P63000 |
| B | RAS-related C3 botulinum toxin substrate 1 isoform RAC1B (EC 3.6.5.2) | protein | 167 | 39% helix, 27% strand | P63000 |
Ligands and binding sites
- GDP · Guanosine-5'-diphosphate · ligand · 2 copies · chains A, B
- MG · Magnesium ion · metal · 2 copies · chains A, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
4 pockets found on a 1 Å grid, 1 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 72 | 0.59 | 0.89 | 16 | empty |
| 2 | 82 | 0.37 | 0.76 | 26 | MG, GDP |
| 3 | 65 | 0.33 | 0.77 | 17 | empty |
| 4 | 64 | 0.30 | 0.75 | 19 | empty |
Interfaces
1 interface between chains.
- A–B · 729 Ų buried · 7 hydrogen bonds · protein-protein
Compared structures
- 1RYF · Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase · the same entry in another collection · chain A against A, TM-score 1.00
- P63000 · AlphaFold monomer V2.0 prediction for RAS-related C3 botulinum toxin substrate 1 (P63000) · a predicted model of the same protein · chain B against A, TM-score 0.96
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P63000
- Elsewhere: RCSB PDB 1RYF · PDBe 1RYF · UniProt P63000
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