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A novel DNA recognition mode by nf-kb P65 homodimer

Analyse A novel DNA recognition mode by nf-kb P65 homodimer in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 1RAM
Type: protein + DNA
Length: 586 residues
Chains: 4
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: DNA (5'- D(*CP*GP*GP*CP*TP*GP*GP*AP*AP*AP*TP*TP*TP*CP*CP*AP*GP*CP*CP *G)-3'); Protein…
UniProt: Q04207
Pockets: 5 · best druggability 0.82

Structure

x-ray diffraction · 2 distinct chains · 10% helix, 40% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
C, D	DNA (5'- D(CPGPGPCPTPGPGPAPAPAPTPTPTPCPCPAPGPCPCP G)-3')	dna	20	—	—
A, B	Protein (transcription factor nf-kb P65)	protein	273	10% helix, 40% strand	Q04207

Ligands and binding sites

DTT · 2,3-dihydroxy-1,4-dithiobutane · additive · 2 copies · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

5 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	1168	0.82	0.84	87	empty
2	577	0.64	0.76	54	empty
3	72	0.37	0.78	13	empty
4	70	0.35	0.77	15	empty
5	50	0.30	0.77	14	empty

Interfaces

6 interfaces between chains.

C–D · 897 Å² buried · 107 hydrogen bonds · nucleic-nucleic
A–B · 704 Å² buried · 9 hydrogen bonds · protein-protein
D–B · 613 Å² buried · 6 hydrogen bonds · protein-nucleic
C–A · 612 Å² buried · 8 hydrogen bonds · protein-nucleic
D–A · 262 Å² buried · 9 hydrogen bonds · protein-nucleic

Compared structures

1RAM · A novel DNA recognition mode by nf-kb P65 homodimer · the same entry in another collection · chain A against A, TM-score 1.00
Q04207 · AlphaFold monomer V2.0 prediction for transcription factor P65 (Q04207) · a predicted model of the same protein · chain A against A, TM-score 0.97

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt Q04207
Elsewhere: RCSB PDB 1RAM · PDBe 1RAM · UniProt Q04207

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