Research workbench
Human DNA polymerase eta-DNA ternary mismatch COMPLEX:REACTION with 10.0 mm MN2+ for 120S
Analyse Human DNA polymerase eta-DNA ternary mismatch COMPLEX:REACTION with 10.0 mm… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7U7G
- Type
- protein + DNA
- Length
- 450 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- DNA polymerase eta; DNA (5'-D(*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3'); DNA…
- EC
- 2.7.7.7
- UniProt
- Q9Y253
- Ligands
- DPO, DGT, MN
- Pockets
- 6 · best druggability 0.75
Structure
x-ray diffraction · 3 distinct chains · 43% helix, 23% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | DNA polymerase eta (EC 2.7.7.7) | protein | 430 | 43% helix, 23% strand | Q9Y253 |
| T | DNA (5'-D(*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3') | dna | 11 | — | — |
| P | DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*TP*G)-3') | dna | 9 | — | — |
Ligands and binding sites
- DPO · Diphosphate · ligand · chains A, P
- DGT · 2'-deoxyguanosine-5'-triphosphate · ligand · chains A, T, P
- MN · Manganese (II) ion · metal · 3 copies · chains A, P
- GOL · Glycerol · additive · 3 copies · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
6 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 545 | 0.75 | 0.83 | 37 | empty |
| 2 | 509 | 0.71 | 0.81 | 51 | empty |
| 3 | 224 | 0.66 | 0.84 | 25 | empty |
| 4 | 268 | 0.52 | 0.75 | 31 | MN, DPO |
| 5 | 92 | 0.42 | 0.79 | 22 | empty |
Interfaces
3 interfaces between chains.
- A–T · 1004 Ų buried · 22 hydrogen bonds · protein-nucleic
- A–P · 820 Ų buried · 18 hydrogen bonds · protein-nucleic
- T–P · 441 Ų buried · 41 hydrogen bonds · nucleic-nucleic
Modifications
Observed in the structure: 1 covalent (covalent ligand).
Compared structures
- 7U7G · Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 10.0 mM Mn2+ for 120s · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q9Y253
- Elsewhere: RCSB PDB 7U7G · PDBe 7U7G · UniProt Q9Y253
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