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Full-length insulin receptor bound with site 2 binding deficient mutant insulin (a-L13R) -- asymmetric conformation

Analyse Full-length insulin receptor bound with site 2 binding deficient mutant insulin… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 7SL2
Type: protein
Length: 1,796 residues
Chains: 10
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Insulin receptor; Insulin B chain; Insulin a chain
EC: 2.7.10.1
UniProt: P15208, P01308
Pockets: 20 · best druggability 0.76

Structure

electron microscopy · 7 distinct chains · 13% helix, 29% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Insulin receptor (EC 2.7.10.1)	protein	806	10% helix, 30% strand	P15208
B	Insulin receptor (EC 2.7.10.1)	protein	798	10% helix, 32% strand	P15208
C	Insulin B chain	protein	27	37% helix, 4% strand	P01308
D	Insulin B chain	protein	25	36% helix, 4% strand	P01308
E	Insulin B chain	protein	27	41% helix, 11% strand	P01308
F	Insulin B chain	protein	29	52% helix, 10% strand	P01308
G, H, I, J	Insulin a chain	protein	21	62% helix, 5% strand	P01308

Pockets

20 pockets found on a 1 Å grid, 14 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	397	0.76	0.86	32	empty
2	176	0.65	0.85	34	empty
3	175	0.63	0.84	30	empty
4	410	0.62	0.77	37	empty
5	476	0.62	0.76	47	empty

Interfaces

25 interfaces between chains.

A–B · 2173 Å² buried · 11 hydrogen bonds · protein-protein
F–J · 796 Å² buried · 8 hydrogen bonds · protein-protein
C–G · 743 Å² buried · 9 hydrogen bonds · protein-protein
B–H · 719 Å² buried · 10 hydrogen bonds · protein-protein
E–I · 692 Å² buried · 7 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 45 disulfide (disulfide).

Compared structures

7SL2 · Full-length insulin receptor bound with site 2 binding deficient mutant insulin (A-L13R)… · the same entry in another collection · chain J against J, TM-score 1.00
P15208 · AlphaFold monomer V2.0 prediction for insulin receptor (P15208) · a predicted model of the same protein · chain A against A, TM-score 0.66

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P15208
Every structure of UniProt P01308
Elsewhere: RCSB PDB 7SL2 · PDBe 7SL2 · UniProt P15208 · UniProt P01308

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