Research workbench
Structure of an inhibited GRK2-G-beta and G-gamma complex
Analyse Structure of an inhibited GRK2-G-beta and G-gamma complex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7PWD
- Type
- protein
- Length
- 1,023 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Beta-adrenergic receptor kinase 1; Guanine nucleotide-binding protein G(I)/G(S)/G(T)…
- EC
- 2.7.11.15
- UniProt
- P25098, P62871, P63212
- Ligands
- 8DS, CL
- Pockets
- 10 · best druggability 0.78
Structure
x-ray diffraction · 3 distinct chains · 33% helix, 25% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Beta-adrenergic receptor kinase 1 (EC 2.7.11.15) | protein | 621 | 45% helix, 15% strand | P25098 |
| B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | protein | 339 | 8% helix, 47% strand | P62871 |
| G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | protein | 63 | 52% helix, 0% strand | P63212 |
Ligands and binding sites
- 8DS · 4-chloranyl-N-[2-(4-chlorophenyl)ethyl]thieno[2,3-c]pyridine-2-carboxamide · ligand · chains A
- CL · Chloride ion · ion · 2 copies · chains A
- 1PE · Pentaethylene glycol · additive · chains A
- GOL · Glycerol · additive · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
10 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 599 | 0.78 | 0.84 | 59 | empty |
| 2 | 317 | 0.63 | 0.79 | 37 | 8DS |
| 3 | 479 | 0.60 | 0.74 | 52 | empty |
| 4 | 236 | 0.56 | 0.78 | 29 | empty |
| 5 | 180 | 0.45 | 0.74 | 22 | empty |
Interfaces
2 interfaces between chains.
- B–G · 2430 Ų buried · 21 hydrogen bonds · protein-protein
- A–B · 1096 Ų buried · 15 hydrogen bonds · protein-protein
Compared structures
- 7PWD · Structure of an inhibited GRK2-G-beta and G-gamma complex · the same entry in another collection · chain A against A, TM-score 1.00
- P62871 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(T)… · a predicted model of the same protein · chain B against A, TM-score 0.99
- P25098 · AlphaFold monomer V2.0 prediction for beta-adrenergic receptor kinase 1 (P25098) · a predicted model of the same protein · chain A against A, TM-score 0.99
- P63212 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(I)/G(S)/G(O)… · a predicted model of the same protein · chain G against A, TM-score 0.61
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P25098
- Every structure of UniProt P62871
- Every structure of UniProt P63212
- Elsewhere: RCSB PDB 7PWD · PDBe 7PWD · UniProt P25098 · UniProt P62871 · UniProt P63212
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