Research workbench
Full integration complex of CAS1/CAS2 from CAS4-containing system
Analyse Full integration complex of CAS1/CAS2 from CAS4-containing system in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7MI9
- Type
- protein + DNA
- Length
- 1,724 residues
- Chains
- 10
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR-associated exonuclease CAS4/ENDONUCLEASE CAS1 fusion; CRISPR-associated…
- EC
- 3.1.-.-, 3.1.12.1
- UniProt
- Q74H36, Q74H35
- Pockets
- 20 · best druggability 0.85
Structure
electron microscopy · 8 distinct chains · 39% helix, 14% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | CRISPR-associated exonuclease CAS4/ENDONUCLEASE CAS1 fusion (EC 3.1.-.-, 3.1.12.1) | protein | 348 | 41% helix, 14% strand | Q74H36 |
| B | CRISPR-associated exonuclease CAS4/ENDONUCLEASE CAS1 fusion (EC 3.1.-.-, 3.1.12.1) | protein | 338 | 39% helix, 11% strand | Q74H36 |
| C, D | CRISPR-associated exonuclease CAS4/ENDONUCLEASE CAS1 fusion (EC 3.1.-.-, 3.1.12.1) | protein | 339 | 43% helix, 15% strand | Q74H36 |
| E, F | CRISPR-associated endoribonuclease CAS2 (EC 3.1.-.-) | protein | 95 | 23% helix, 26% strand | Q74H35 |
| G | DNA (80-mer) | dna | 80 | — | — |
| H | DNA (72-mer) | dna | 72 | — | — |
| I | DNA (5'-D(P*CP*GP*GP*AP*AP*AP*AP*GP*AP*GP*CP*C)-3') | dna | 12 | — | — |
| J | DNA (5'-D(P*GP*AP*AP*GP*CP*C)-3') | dna | 6 | — | — |
Pockets
20 pockets found on a 1 Å grid, 14 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 939 | 0.85 | 0.86 | 115 | empty |
| 2 | 672 | 0.79 | 0.84 | 64 | empty |
| 3 | 998 | 0.78 | 0.80 | 88 | empty |
| 4 | 706 | 0.75 | 0.81 | 76 | empty |
| 5 | 440 | 0.72 | 0.82 | 60 | empty |
Interfaces
23 interfaces between chains.
- G–H · 2817 Ų buried · 217 hydrogen bonds · nucleic-nucleic
- A–B · 1810 Ų buried · 21 hydrogen bonds · protein-protein
- C–D · 1704 Ų buried · 19 hydrogen bonds · protein-protein
- E–F · 1442 Ų buried · 18 hydrogen bonds · protein-protein
- B–H · 1249 Ų buried · 25 hydrogen bonds · protein-nucleic
Compared structures
- 7MI9 · Full integration complex of Cas1/Cas2 from Cas4-containing system · the same entry in another collection · chain J against J, TM-score 1.00
- Q74H36 · AlphaFold monomer V2.0 prediction for CRISPR-associated exonuclease CAS4/ENDONUCLEASE… · a predicted model of the same protein · chain A against A, TM-score 0.95
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q74H36
- Every structure of UniProt Q74H35
- Elsewhere: RCSB PDB 7MI9 · PDBe 7MI9 · UniProt Q74H36 · UniProt Q74H35
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