Research workbench
Bovine cardiac myosin in complex with chicken skeletal actin and human cardiac tropomyosin in the rigor state
Analyse Bovine cardiac myosin in complex with chicken skeletal actin and human cardiac… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6X5Z
- Type
- protein
- Length
- 3,554 residues
- Chains
- 8
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Actin, alpha skeletal muscle; Tropomyosin alpha-1 chain; Myosin-7
- UniProt
- P68139, P09493, Q9BE39
- Ligands
- ADP, MG
- Pockets
- 20 · best druggability 0.85
Structure
electron microscopy · 3 distinct chains · 49% helix, 13% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| B, A, C | Actin, alpha skeletal muscle | protein | 369 | 40% helix, 18% strand | P68139 |
| O, P | Tropomyosin alpha-1 chain | protein | 166 | 99% helix, 0% strand | P09493 |
| G, D, J | Myosin-7 | protein | 705 | 43% helix, 13% strand | Q9BE39 |
Ligands and binding sites
- ADP · Adenosine-5'-diphosphate · ligand · 3 copies · chains B, A, C
- MG · Magnesium ion · metal · 3 copies · chains B, A, C
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 977 | 0.85 | 0.86 | 101 | empty |
| 2 | 1017 | 0.84 | 0.85 | 84 | empty |
| 3 | 619 | 0.82 | 0.87 | 80 | ADP, MG |
| 4 | 1176 | 0.81 | 0.82 | 95 | empty |
| 5 | 411 | 0.80 | 0.89 | 54 | ADP, MG |
Interfaces
20 interfaces between chains.
- O–P · 4436 Ų buried · 7 hydrogen bonds · protein-protein
- B–A · 1160 Ų buried · 11 hydrogen bonds · protein-protein
- B–C · 1133 Ų buried · 11 hydrogen bonds · protein-protein
- A–D · 1070 Ų buried · 13 hydrogen bonds · protein-protein
- B–G · 1069 Ų buried · 13 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 3 covalent (covalent ligand).
Compared structures
- 6X5Z · Bovine Cardiac Myosin in Complex with Chicken Skeletal Actin and Human Cardiac… · the same entry in another collection · chain J against J, TM-score 1.00
- P68139 · AlphaFold monomer V2.0 prediction for actin, alpha skeletal muscle (P68139) · a predicted model of the same protein · chain B against A, TM-score 0.95
- Q9BE39 · AlphaFold monomer V2.0 prediction for myosin-7 (Q9BE39) · a predicted model of the same protein · chain G against A, TM-score 0.82
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P68139
- Every structure of UniProt P09493
- Every structure of UniProt Q9BE39
- Elsewhere: RCSB PDB 6X5Z · PDBe 6X5Z · UniProt P68139 · UniProt P09493 · UniProt Q9BE39
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.