Research workbench
Human DNA polymerase eta extension complex with cda at the -1 position
Analyse Human DNA polymerase eta extension complex with cda at the -1 position in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6M7V
- Type
- protein + DNA
- Length
- 439 residues
- Chains
- 3
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- DNA polymerase eta; DNA (5'-D(P*GP*TP*GP*TP*GP*AP*GP*T)-3'); DNA…
- EC
- 2.7.7.7
- UniProt
- Q9Y253
- Ligands
- DZ4, MN, NI
- Pockets
- 4 · best druggability 0.82
Structure
x-ray diffraction · 3 distinct chains · 43% helix, 21% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | DNA polymerase eta (EC 2.7.7.7) | protein | 422 | 43% helix, 21% strand | Q9Y253 |
| P | DNA (5'-D(P*GP*TP*GP*TP*GP*AP*GP*T)-3') | dna | 8 | — | — |
| T | DNA (5'-D(P*(02I)P*CP*TP*CP*AP*CP*AP*CP*T)-3') | dna | 9 | — | — |
Ligands and binding sites
- DZ4 · 2'-deoxy-5'-O-[(R)-hydroxy{[(r)-hydroxy(phosphonooxy) phosphoryl]amino}phosphoryl]adenosine · ligand · chains A, P
- MN · Manganese (II) ion · metal · 2 copies · chains A, P
- NI · Nickel (II) ion · metal · chains A
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
4 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 970 | 0.82 | 0.83 | 87 | DZ4, MN |
| 2 | 559 | 0.73 | 0.81 | 39 | empty |
| 3 | 73 | 0.48 | 0.83 | 15 | empty |
| 4 | 80 | 0.36 | 0.76 | 19 | empty |
Interfaces
3 interfaces between chains.
- A–T · 524 Ų buried · 13 hydrogen bonds · protein-nucleic
- A–P · 518 Ų buried · 11 hydrogen bonds · protein-nucleic
- P–T · 368 Ų buried · 35 hydrogen bonds · nucleic-nucleic
Modifications
Observed in the structure: 1 modified (nucleotide modification).
Compared structures
- 6M7V · Human DNA polymerase eta extension complex with cdA at the -1 position · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q9Y253
- Elsewhere: RCSB PDB 6M7V · PDBe 6M7V · UniProt Q9Y253
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