Research workbench
Cryo-EM structure of human insulin degrading enzyme in complex with insulin
Analyse Cryo-EM structure of human insulin degrading enzyme in complex with insulin in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 6BFC
- Type
- protein
- Length
- 1,935 residues
- Chains
- 4
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Insulin-degrading enzyme; Insulin
- EC
- 3.4.24.56
- UniProt
- P14735, P01308
- Pockets
- 20 · best druggability 0.87
Structure
electron microscopy · 4 distinct chains · 37% helix, 17% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Insulin-degrading enzyme (EC 3.4.24.56) | protein | 948 | 38% helix, 17% strand | P14735 |
| B | Insulin-degrading enzyme (EC 3.4.24.56) | protein | 938 | 39% helix, 17% strand | P14735 |
| a | Insulin | protein | 27 | 0% helix, 0% strand | P01308 |
| b | Insulin | protein | 22 | 0% helix, 5% strand | P01308 |
Pockets
20 pockets found on a 1 Å grid, 15 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 799 | 0.87 | 0.89 | 83 | empty |
| 2 | 1002 | 0.86 | 0.87 | 98 | empty |
| 3 | 1050 | 0.84 | 0.85 | 98 | empty |
| 4 | 934 | 0.84 | 0.85 | 126 | empty |
| 5 | 629 | 0.76 | 0.82 | 78 | empty |
Interfaces
3 interfaces between chains.
- A–B · 1552 Ų buried · 20 hydrogen bonds · protein-protein
- A–a · 1544 Ų buried · 13 hydrogen bonds · protein-protein
- B–b · 1216 Ų buried · 11 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 2 disulfide (disulfide).
Compared structures
- 6BFC · Cryo-EM structure of human insulin degrading enzyme in complex with insulin · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P14735
- Every structure of UniProt P01308
- Elsewhere: RCSB PDB 6BFC · PDBe 6BFC · UniProt P14735 · UniProt P01308
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