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Crystal structure of gamma-chymotrypsin at pH 5.6, room temperature

Analyse Crystal structure of gamma-chymotrypsin at pH 5.6, room temperature in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 5R48
Type: protein
Length: 245 residues
Chains: 5
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Gamma-chymotrypsin; Peptide swpw; Peptide tpgvy
EC: 3.4.21.1
UniProt: P00766
Ligands: IOD, SO4

Structure

x-ray diffraction · 5 distinct chains · 11% helix, 39% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Gamma-chymotrypsin (EC 3.4.21.1)	protein	10	0% helix, 0% strand	P00766
B	Gamma-chymotrypsin (EC 3.4.21.1)	protein	131	2% helix, 40% strand	P00766
C	Gamma-chymotrypsin (EC 3.4.21.1)	protein	95	25% helix, 40% strand	P00766
D	Peptide swpw	protein	4	0% helix, 50% strand	—
E	Peptide tpgvy	protein	5	0% helix, 60% strand	—

Ligands and binding sites

IOD · Iodide ion · ion · chains B
SO4 · Sulfate ion · ion · chains B, C

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

8 interfaces between chains.

B–C · 2927 Å² buried · 40 hydrogen bonds · protein-protein
A–B · 472 Å² buried · 1 hydrogen bond · protein-protein
C–E · 394 Å² buried · 10 hydrogen bonds · protein-protein
C–D · 375 Å² buried · 9 hydrogen bonds · protein-protein
D–E · 302 Å² buried · 23 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 5 disulfide (disulfide).

Compared structures

5R48 · Crystal Structure of gamma-Chymotrypsin at pH 5.6, room temperature · the same entry in another collection · chain E against E, TM-score 1.00
P00766 · AlphaFold monomer V2.0 prediction for chymotrypsinogen a (P00766) · a predicted model of the same protein · chain C against A, TM-score 0.99

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P00766
Elsewhere: RCSB PDB 5R48 · PDBe 5R48 · UniProt P00766

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