Research workbench
Crystal structure of yeast BRR2 full-lenght in complex with PRP8 JAB1 domain
Analyse Crystal structure of yeast BRR2 full-lenght in complex with PRP8 JAB1 domain in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5M52
- Type
- protein
- Length
- 4,306 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Pre-mRNA-splicing helicase BRR2; Pre-mRNA-splicing factor 8
- EC
- 3.6.4.13
- UniProt
- P32639, P33334
- Pockets
- 20 · best druggability 0.86
Structure
x-ray diffraction · 4 distinct chains · 41% helix, 17% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Pre-mRNA-splicing helicase BRR2 (EC 3.6.4.13) | protein | 1910 | 45% helix, 15% strand | P32639 |
| C | Pre-mRNA-splicing factor 8 | protein | 266 | 18% helix, 26% strand | P33334 |
| B | Pre-mRNA-splicing helicase BRR2 (EC 3.6.4.13) | protein | 1862 | 44% helix, 16% strand | P32639 |
| D | Pre-mRNA-splicing factor 8 | protein | 268 | 21% helix, 25% strand | P33334 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1117 | 0.86 | 0.87 | 98 | empty |
| 2 | 1046 | 0.86 | 0.87 | 96 | empty |
| 3 | 1071 | 0.84 | 0.85 | 96 | empty |
| 4 | 556 | 0.83 | 0.89 | 87 | empty |
| 5 | 1103 | 0.83 | 0.84 | 92 | empty |
Interfaces
4 interfaces between chains.
- A–C · 2896 Ų buried · 30 hydrogen bonds · protein-protein
- B–D · 2837 Ų buried · 34 hydrogen bonds · protein-protein
- A–B · 1074 Ų buried · 15 hydrogen bonds · protein-protein
- C–B · 544 Ų buried · 7 hydrogen bonds · protein-protein
Compared structures
- 5M52 · Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain · the same entry in another collection · chain A against A, TM-score 1.00
- P32639 · AlphaFold monomer V2.0 prediction for pre-mRNA-splicing helicase BRR2 (P32639) · a predicted model of the same protein · chain A against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P32639
- Every structure of UniProt P33334
- Elsewhere: RCSB PDB 5M52 · PDBe 5M52 · UniProt P32639 · UniProt P33334
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.