Research workbench
Saccharomyces cerevisiae acetohydroxyacid synthase in complex with bensulfuron methyl
Analyse Saccharomyces cerevisiae acetohydroxyacid synthase in complex with bensulfuron… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 5FEM
- Type
- protein
- Length
- 1,198 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Acetolactate synthase catalytic subunit, mitochondrial
- EC
- 2.2.1.6
- UniProt
- P07342
- Ligands
- FAD, 60G, TPP, MG
- Pockets
- 13 · best druggability 0.86
Structure
x-ray diffraction · 1 distinct chain · 44% helix, 17% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, B | Acetolactate synthase catalytic subunit, mitochondrial (EC 2.2.1.6) | protein | 599 | 44% helix, 17% strand | P07342 |
Ligands and binding sites
- FAD · Flavin-adenine dinucleotide · ligand · 2 copies · chains A, B
- 60G · methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate · ligand · 2 copies · chains A, B
- TPP · Thiamine diphosphate · ligand · 2 copies · chains A, B
- MG · Magnesium ion · metal · 4 copies · chains A, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
13 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 925 | 0.86 | 0.88 | 113 | MG, FAD, 60G, TPP |
| 2 | 910 | 0.86 | 0.88 | 118 | FAD, MG, 60G, TPP |
| 3 | 107 | 0.61 | 0.87 | 22 | empty |
| 4 | 175 | 0.58 | 0.82 | 34 | empty |
| 5 | 175 | 0.57 | 0.81 | 28 | empty |
Interfaces
1 interface between chains.
- A–B · 3224 Ų buried · 38 hydrogen bonds · protein-protein
Compared structures
- 5FEM · Saccharomyces cerevisiae Acetohydroxyacid Synthase in complex with bensulfuron methyl · the same entry in another collection · chain A against A, TM-score 1.00
- P07342 · AlphaFold monomer V2.0 prediction for acetolactate synthase catalytic subunit… · a predicted model of the same protein · chain A against A, TM-score 0.99
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P07342
- Elsewhere: RCSB PDB 5FEM · PDBe 5FEM · UniProt P07342
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.