Research workbench

Human MHF1-MHF2 complex

Analyse Human MHF1-MHF2 complex in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 4NE5
Type: protein
Length: 668 residues
Chains: 8
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Centromere protein S; Centromere protein X
UniProt: Q8N2Z9, A8MT69
Pockets: 7 · best druggability 0.75

Structure

x-ray diffraction · 2 distinct chains · 75% helix, 5% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A, C, E, G	Centromere protein S	protein	93	81% helix, 4% strand	Q8N2Z9
B, D, F, H	Centromere protein X	protein	74	69% helix, 5% strand	A8MT69

Pockets

7 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	988	0.75	0.78	76	empty
2	185	0.54	0.79	20	empty
3	170	0.53	0.79	20	empty
4	139	0.49	0.79	19	empty
5	141	0.43	0.75	21	empty

Interfaces

18 interfaces between chains.

G–H · 2408 Å² buried · 17 hydrogen bonds · protein-protein
C–D · 2400 Å² buried · 18 hydrogen bonds · protein-protein
A–B · 2396 Å² buried · 17 hydrogen bonds · protein-protein
E–F · 2394 Å² buried · 16 hydrogen bonds · protein-protein
C–G · 549 Å² buried · 11 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 12 modified (engineered).

Compared structures

4NE5 · Human MHF1-MHF2 complex · the same entry in another collection · chain H against H, TM-score 1.00
A8MT69 · AlphaFold monomer V2.0 prediction for centromere protein X (A8MT69) · a predicted model of the same protein · chain D against A, TM-score 0.99

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt Q8N2Z9
Every structure of UniProt A8MT69
Elsewhere: RCSB PDB 4NE5 · PDBe 4NE5 · UniProt Q8N2Z9 · UniProt A8MT69

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