Crystal structure of disulfide-bond-crosslinked complex of bovine hsc70 (1-394aa)R171C and bovine Auxilin (810-910aa)D876C in the AMPPNP hydrolyzed form

Structure

x-ray diffraction · resolution 2.21 · 2 distinct chains · 43% helix, 23% strand (DSSP).

Ligands and binding sites

• ADP · Adenosine-5'-diphosphate · ligand · chains A
• PO4 · Phosphate ion · ion · chains A
• GOL · Glycerol · additive · 2 copies · chains A
• ACY · Acetic acid · additive · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

3 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 451 Ų buried · 2 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 1 disulfide (disulfide).

Compared structures

• 2QWQ · Crystal structure of disulfide-bond-crosslinked complex of bovine HSC70 (1-394AA)R171C… · the same entry in another collection · chain A against A, TM-score 1.00
• Q27974 · AlphaFold monomer V2.0 prediction for putative tyrosine-protein phosphatase auxilin… · a predicted model of the same protein · chain B against A, TM-score 0.98
• P19120 · AlphaFold monomer V2.0 prediction for heat shock cognate 71 kda protein (P19120) · a predicted model of the same protein · chain A against A, TM-score 0.90

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P19120
• Every structure of UniProt Q27974
• Elsewhere: RCSB PDB 2QWQ · PDBe 2QWQ · UniProt P19120 · UniProt Q27974