Research workbench
Human PrimPol misinserting dATP opposite the 8-oxoguanine lesion (3'-end base of the primer strand is 2',3'-dideoxy-terminated).
Analyse Human PrimPol misinserting dATP opposite the 8-oxoguanine lesion (3'-end base… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 7JKL
- Type
- protein + DNA
- Length
- 576 residues
- Chains
- 6
- Method
- x-ray diffraction
- Collection
- MolNFT v2 / PDB Bank
- Contract
- 0xd58B01f6C18086e5202cdC5D7Ad3E41790360102
- Owner
- 0xF4e1AA6f26B7DA48EeAD9B24a97927303eeA07b8
- Resolution
- 2.384
- Source
- Homo sapiens; SYNTHETIC CONSTRUCT
- Molecule
- DNA-directed primase/polymerase protein; DNA (5'-D(P*AP*(8OG)P*CP*GP*CP*TP*AP*CP*CP*AP*CP*…
- EC
- 2.7.7.-
- UniProt
- Q96LW4
- Ligands
- DTP, CA
- Pockets
- 7 · best druggability 0.72
Structure
x-ray diffraction · resolution 2.384 · 5 distinct chains · 29% helix, 23% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | DNA-directed primase/polymerase protein (EC 2.7.7.-) | protein | 269 | 29% helix, 22% strand | Q96LW4 |
| C, G | DNA (5'-D(P*AP*(8OG)P*CP*GP*CP*TP*AP*CP*CP*AP*CP*AP*CP*CP*CP*C)-3') | dna | 16 | — | — |
| D | DNA (5'-D(*GP*GP*GP*TP*GP*TP*GP*GP*TP*AP*GP*CP*(DDG))-3') | dna | 13 | — | — |
| B | DNA-directed primase/polymerase protein (EC 2.7.7.-) | protein | 251 | 28% helix, 23% strand | Q96LW4 |
| H | DNA (5'-D(*GP*GP*GP*TP*GP*TP*GP*GP*TP*AP*GP*CP*(DDG))-3') | dna | 11 | — | — |
Ligands and binding sites
- DTP · 2'-deoxyadenosine 5'-triphosphate · ligand · 2 copies · chains A, C, D, B, G, H
- CA · Calcium ion · metal · 3 copies · chains A, B, H
- GOL · Glycerol · additive · 2 copies · chains A, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
7 pockets found on a 1 Å grid, 2 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 753 | 0.72 | 0.79 | 61 | DTP, CA |
| 2 | 639 | 0.69 | 0.78 | 56 | CA, DTP |
| 3 | 150 | 0.47 | 0.77 | 28 | empty |
| 4 | 148 | 0.44 | 0.76 | 21 | empty |
| 5 | 87 | 0.38 | 0.77 | 25 | empty |
Interfaces
7 interfaces between chains.
- A–B · 630 Ų buried · 13 hydrogen bonds · protein-protein
- C–D · 623 Ų buried · 56 hydrogen bonds · nucleic-nucleic
- A–C · 571 Ų buried · 12 hydrogen bonds · protein-nucleic
- G–H · 533 Ų buried · 52 hydrogen bonds · nucleic-nucleic
- B–G · 512 Ų buried · 13 hydrogen bonds · protein-nucleic
Modifications
Observed in the structure: 4 modified (nucleotide modification).
Compared structures
- 7JKL · Human primpol misinserting datp opposite the 8-oxoguanine lesion (3'- end base of the… · the same entry in another collection · chain H against H, TM-score 1.00
- Q96LW4 · AlphaFold monomer V2.0 prediction for DNA-directed PRIMASE/POLYMERASE protein (Q96LW4) · a predicted model of the same protein · chain B against A, TM-score 0.99
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q96LW4
- Elsewhere: RCSB PDB 7JKL · PDBe 7JKL · UniProt Q96LW4
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