Crystal structure of the C. elegans Protein O-fucosyltransferase 2 (CePOFUT2) double mutant (R298K-R299K) in complex with GDP and the human TSR1 from thrombospondin 1

Structure

x-ray diffraction · resolution 1.98 · 2 distinct chains · 34% helix, 21% strand (DSSP).

Ligands and binding sites

• GDP · Guanosine-5'-diphosphate · ligand · 2 copies · chains A, B
• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 2 copies · chains A, B
• EDO · 1,2-ethanediol · additive · 10 copies · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

13 pockets found on a 1 Å grid, 11 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 784 Ų buried · 10 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 10 disulfide, 2 glycan (disulfide, N-glycosylation).

Compared structures

• 5FOE · Crystal structure of the C. elegans protein O-fucosyltransferase 2 (CEPOFUT2) double… · the same entry in another collection · chain A against A, TM-score 1.00
• Q8WR51 · AlphaFold monomer V2.0 prediction for GDP-fucose protein O-fucosyltransferase 2 (Q8WR51) · a predicted model of the same protein · chain B against A, TM-score 0.88
• P07996 · AlphaFold monomer V2.0 prediction for thrombospondin-1 (P07996) · a predicted model of the same protein · chain A against A, TM-score 0.29

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt Q8WR51
• Every structure of UniProt P07996
• Elsewhere: RCSB PDB 5FOE · PDBe 5FOE · UniProt Q8WR51 · UniProt P07996