Crystal structure of a RAS P21 protein activator (RASA1) from homo sapiens at 2.25 a resolution

Structure

x-ray diffraction · 2 distinct chains · 5% helix, 47% strand (DSSP).

Ligands and binding sites

• CL · Chloride ion · ion · 3 copies · chains A, B, C
• PO4 · Phosphate ion · ion · chains A, B
• GOL · Glycerol · additive · chains C

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Interfaces

2 interfaces between chains.

• A–C · 443 Ų buried · 11 hydrogen bonds · protein-protein
• A–B · 390 Ų buried · 8 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 6 modified (engineered).

Compared structures

• 4FSS · Crystal structure of a RAS p21 protein activator (RASA1) from Homo sapiens at 2.25 A… · the same entry in another collection · chain A against A, TM-score 1.00
• P20936 · AlphaFold monomer V2.0 prediction for RAS GTPase-activating protein 1 (P20936) · a predicted model of the same protein · chain B against A, TM-score 0.96

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P20936
• Elsewhere: RCSB PDB 4FSS · PDBe 4FSS · UniProt P20936