MolNFT v1 · #8011 · protein

Crystal structure of MAD1-MAD2 reveals a conserved MAD2 binding motif in MAD1 and CDC20.

1GO4 · Mitotic spindle assembly checkpoint protein MAD2A: “Crystal structure of MAD1-MAD2 reveals a conserved MAD2 binding motif in MAD1 and CDC20.”. X-ray diffraction, 15 pockets (best druggability 0.77). MolNFT v1 #8011 on GenesisL1: 3D…

PDB: 1GO4
Type: protein
Length: 1,146 residues
Chains: 8
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Mitotic spindle assembly checkpoint protein MAD2A; Mitotic spindle assembly checkpoint…
UniProt: Q13257, Q9Y6D9
Pockets: 15 · best druggability 0.77

Structure

x-ray diffraction · 6 distinct chains · 46% helix, 25% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Mitotic spindle assembly checkpoint protein MAD2A	protein	196	35% helix, 33% strand	Q13257
B, C	Mitotic spindle assembly checkpoint protein MAD2A	protein	195	35% helix, 35% strand	Q13257
D	Mitotic spindle assembly checkpoint protein MAD2A	protein	193	34% helix, 34% strand	Q13257
E, F	Mitotic spindle assembly checkpoint protein MAD1	protein	87	71% helix, 7% strand	Q9Y6D9
G	Mitotic spindle assembly checkpoint protein MAD1	protein	100	71% helix, 8% strand	Q9Y6D9
H	Mitotic spindle assembly checkpoint protein MAD1	protein	93	71% helix, 8% strand	Q9Y6D9

Pockets

15 pockets found on a 1 Å grid, 4 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	964	0.77	0.79	103	empty
2	1169	0.75	0.78	102	empty
3	351	0.56	0.75	38	empty
4	299	0.54	0.75	44	empty
5	59	0.50	0.86	18	empty

Interfaces

19 interfaces between chains.

G–H · 1602 Å² buried · 24 hydrogen bonds · protein-protein
B–F · 1547 Å² buried · 41 hydrogen bonds · protein-protein
E–F · 1512 Å² buried · 23 hydrogen bonds · protein-protein
A–G · 1487 Å² buried · 32 hydrogen bonds · protein-protein
D–H · 1421 Å² buried · 26 hydrogen bonds · protein-protein

Compared structures

1GO4 · Crystal structure of Mad1-Mad2 reveals a conserved Mad2 binding motif in Mad1 and Cdc20. · the same entry in another collection · chain H against H, TM-score 1.00
Q13257 · AlphaFold monomer V2.0 prediction for mitotic spindle assembly checkpoint protein MAD2A… · a predicted model of the same protein · chain B against A, TM-score 0.98
Q9Y6D9 · AlphaFold monomer V2.0 prediction for mitotic spindle assembly checkpoint protein MAD1… · a predicted model of the same protein · chain H against A, TM-score 0.55

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt Q13257
Every structure of UniProt Q9Y6D9
Elsewhere: RCSB PDB 1GO4 · PDBe 1GO4 · UniProt Q13257 · UniProt Q9Y6D9

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