Crystal structure of ubiquitin-conjugating enzyme E2-25KDA (huntington interacting protein 2) M172A mutant crystallized at pH 8.5

Structure

x-ray diffraction · 1 distinct chain · 45% helix, 13% strand (DSSP).

Ligands and binding sites

• CA · Calcium ion · metal · chains A
• PG4 · Tetraethylene glycol · additive · 2 copies · chains A
• BME · Beta-mercaptoethanol · additive · chains A
• TRS · 2-amino-2-hydroxymethyl-propane-1,3-diol · additive · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Modifications

Observed in the structure: 1 covalent (additive adduct).

Compared structures

• 3F92 · Crystal structure of ubiquitin-conjugating enzyme E2-25kDa (Huntington Interacting… · the same entry in another collection · chain A against A, TM-score 1.00
• P61086 · AlphaFold monomer V2.0 prediction for ubiquitin-conjugating enzyme E2 K (P61086) · a predicted model of the same protein · chain A against A, TM-score 0.99

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P61086
• Elsewhere: RCSB PDB 3F92 · PDBe 3F92 · UniProt P61086