MolNFT v1 · #26548 · protein
Crystal structure of E.COLI RNase hi active site mutant (E48A/K87A/D134N)
1WSI · Ribonuclease hi: “Crystal structure of E.COLI RNase hi active site mutant (E48A/K87A/D134N)”. X-ray diffraction, 1 pocket (best druggability 0.68). MolNFT v1 #26548 on GenesisL1: 3D structure, binding sites and pockets in GL1X.
- PDB
- 1WSI
- Type
- protein
- Length
- 606 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Ribonuclease hi
- EC
- 3.1.26.4
- UniProt
- P0A7Y4
- Pockets
- 1 · best druggability 0.68
Structure
x-ray diffraction · 4 distinct chains · 36% helix, 32% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Ribonuclease hi (EC 3.1.26.4) | protein | 152 | 36% helix, 30% strand | P0A7Y4 |
| B | Ribonuclease hi (EC 3.1.26.4) | protein | 149 | 36% helix, 34% strand | P0A7Y4 |
| C | Ribonuclease hi (EC 3.1.26.4) | protein | 153 | 36% helix, 31% strand | P0A7Y4 |
| D | Ribonuclease hi (EC 3.1.26.4) | protein | 152 | 37% helix, 33% strand | P0A7Y4 |
Pockets
1 pocket found on a 1 Å grid, 1 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 583 | 0.68 | 0.78 | 59 | empty |
Interfaces
1 interface between chains.
- C–D · 739 Ų buried · 9 hydrogen bonds · protein-protein
Compared structures
- 1WSI · Crystal structure of E.coli RNase HI active site mutant (E48A/K87A/D134N) · the same entry in another collection · chain D against D, TM-score 1.00
- P0A7Y4 · AlphaFold monomer V2.0 prediction for ribonuclease hi (P0A7Y4) · a predicted model of the same protein · chain B against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P0A7Y4
- Elsewhere: RCSB PDB 1WSI · PDBe 1WSI · UniProt P0A7Y4
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