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Crystal structure of E.COLI RNase hi active site mutant (E48A/K87A/D134N)

Analyse Crystal structure of E.COLI RNase hi active site mutant (E48A/K87A/D134N) in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB
1WSI
Type
protein
Length
606 residues
Chains
4
Method
x-ray diffraction
Collection
MolNFT v1 / PDB
Contract
0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner
0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule
Ribonuclease hi
EC
3.1.26.4
UniProt
P0A7Y4
Pockets
1 · best druggability 0.68

Structure

x-ray diffraction · 4 distinct chains · 36% helix, 32% strand (DSSP).

ChainMoleculeTypeResiduesSecondary structureUniProt
ARibonuclease hi (EC 3.1.26.4)protein15236% helix, 30% strandP0A7Y4
BRibonuclease hi (EC 3.1.26.4)protein14936% helix, 34% strandP0A7Y4
CRibonuclease hi (EC 3.1.26.4)protein15336% helix, 31% strandP0A7Y4
DRibonuclease hi (EC 3.1.26.4)protein15237% helix, 33% strandP0A7Y4

Pockets

1 pocket found on a 1 Å grid, 1 with a druggability of 0.5 or more.

PocketVolume (ų)DruggabilityBuriednessLining residuesInside
15830.680.7859empty

Interfaces

1 interface between chains.

  • C–D · 739 Ų buried · 9 hydrogen bonds · protein-protein

Compared structures

  • 1WSI · Crystal structure of E.coli RNase HI active site mutant (E48A/K87A/D134N) · the same entry in another collection · chain D against D, TM-score 1.00
  • P0A7Y4 · AlphaFold monomer V2.0 prediction for ribonuclease hi (P0A7Y4) · a predicted model of the same protein · chain B against A, TM-score 0.98

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