The 2.0 crystal structure of EQFP611, a far-red fluorescent protein from the sea anemone entacmaea quadricolor

Structure

x-ray diffraction · 1 distinct chain · 4% helix, 56% strand (DSSP).

Ligands and binding sites

• NRQ · {(4Z)-4-(4-hydroxybenzylidene)-2-[3-(methylthio) propanimidoyl]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid · ligand · 2 copies · chains A, B
• CA · Calcium ion · metal · 2 copies · chains A, B
• ACY · Acetic acid · additive · 2 copies · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

3 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 1271 Ų buried · 13 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 4 cofactor (chromophore).

Compared structures

• 1UIS · The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone… · the same entry in another collection · chain A against A, TM-score 1.00
• Q8ISF8 · AlphaFold monomer V2.0 prediction for red fluorescent protein EQFP611 (Q8ISF8) · a predicted model of the same protein · chain A against A, TM-score 0.98

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt Q8ISF8
• Elsewhere: RCSB PDB 1UIS · PDBe 1UIS · UniProt Q8ISF8