Cryoem structure of human low-voltage activated T-type calcium channel CAV3.3 in complex with otilonium BROMIDE(OB)

Structure

electron microscopy · 1 distinct chain · 67% helix, 1% strand (DSSP).

Ligands and binding sites

• 3PE · 1,2-diacyl-sn-glycero-3-phosphoethanolamine · ligand · 6 copies · chains A
• Y01 · Cholesterol hemisuccinate · ligand · 6 copies · chains A
• 7TB · 2-[diethyl(methyl)-$l^{4}-azanyl]ethyl 4-[(2-octoxyphenyl)carbonylamino]benzoate · ligand · chains A
• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 2 copies · chains A
• CA · Calcium ion · metal · 2 copies · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 11 with a druggability of 0.5 or more.

Modifications

Observed in the structure: 5 disulfide, 2 glycan, 1 covalent (disulfide, N-glycosylation, covalent ligand).

Compared structures

• 7WLK · CryoEM structure of human low-voltage activated T-type calcium channel Cav3.3 in complex… · the same entry in another collection · chain A against A, TM-score 1.00
• Q9P0X4 · AlphaFold monomer V2.0 prediction for voltage-dependent T-type calcium channel subunit… · a predicted model of the same protein · chain A against A, TM-score 0.98

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt Q9P0X4
• Elsewhere: RCSB PDB 7WLK · PDBe 7WLK · UniProt Q9P0X4