MolNFT v1 · #189300 · protein + DNA + RNA

Catalytic core of human telomerase holoenzyme

7V99 · Telomerase reverse transcriptase: “Catalytic core of human telomerase holoenzyme”. Electron microscopy, 20 pockets (best druggability 0.84). MolNFT v1 #189300 on GenesisL1: 3D structure, binding sites and pockets in GL1X.

PDB: 7V99
Type: protein + DNA + RNA
Length: 1,419 residues
Chains: 5
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Telomerase reverse transcriptase; Histone H2A type 1-B/E; Histone H2B type 1-K…
EC: 2.7.7.49
UniProt: O14746, P04908, O60814
Pockets: 20 · best druggability 0.84

Structure

electron microscopy · 5 distinct chains · 49% helix, 12% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	Telomerase reverse transcriptase (EC 2.7.7.49)	protein	991	46% helix, 13% strand	O14746
K	Histone H2A type 1-B/E	protein	85	66% helix, 5% strand	P04908
L	Histone H2B type 1-K	protein	94	73% helix, 4% strand	O60814
R	Telomerase RNA component	rna	243	—	—
S	Primer DNA	dna	6	—	—

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	945	0.84	0.85	78	empty
2	1002	0.81	0.83	92	empty
3	1178	0.81	0.82	107	empty
4	398	0.80	0.89	49	empty
5	834	0.78	0.82	74	empty

Interfaces

6 interfaces between chains.

A–R · 5853 Å² buried · 89 hydrogen bonds · protein-nucleic
K–L · 2341 Å² buried · 12 hydrogen bonds · protein-protein
K–R · 787 Å² buried · 14 hydrogen bonds · protein-nucleic
A–S · 661 Å² buried · 5 hydrogen bonds · protein-nucleic
L–R · 639 Å² buried · 9 hydrogen bonds · protein-nucleic

Modifications

Observed in the structure: 2 disulfide (disulfide).

Compared structures

7V99 · catalytic core of human telomerase holoenzyme · the same entry in another collection · chain S against S, TM-score 1.00
O14746 · AlphaFold monomer V2.0 prediction for telomerase reverse transcriptase (O14746) · a predicted model of the same protein · chain A against A, TM-score 0.89
P04908 · AlphaFold monomer V2.0 prediction for histone H2A type 1-B/E (P04908) · a predicted model of the same protein · chain K against A, TM-score 0.87
O60814 · AlphaFold monomer V2.0 prediction for histone H2B type 1-K (O60814) · a predicted model of the same protein · chain L against A, TM-score 0.79

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt O14746
Every structure of UniProt P04908
Every structure of UniProt O60814
Elsewhere: RCSB PDB 7V99 · PDBe 7V99 · UniProt O14746 · UniProt P04908 · UniProt O60814

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