Complex gggg of AMPA-subtype iglur GLUA2 in complex with auxiliary subunit GAMMA2 (stargazin) at low glutamate concentration (20 um) in the presence of cyclothiazide (100 um)

Structure

electron microscopy · 2 distinct chains · 48% helix, 12% strand (DSSP).

Ligands and binding sites

• GLU · Glutamic acid · ligand · 4 copies · chains A, B, C, D
• CYZ · Cyclothiazide · ligand · 4 copies · chains A, D, B, C

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Interfaces

4 interfaces between chains.

• B–C · 3743 Ų buried · 20 hydrogen bonds · protein-protein
• A–D · 3630 Ų buried · 20 hydrogen bonds · protein-protein
• A–B · 2848 Ų buried · 18 hydrogen bonds · protein-protein
• C–D · 2833 Ų buried · 16 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 12 disulfide (disulfide).

Compared structures

• 7TNP · Complex GGGG of AMPA-subtype iGluR GluA2 in complex with auxiliary subunit gamma2… · the same entry in another collection · chain D against D, TM-score 1.00
• P19491 · AlphaFold monomer V2.0 prediction for glutamate receptor 2 (P19491) · a predicted model of the same protein · chain D against A, TM-score 0.64
• Q8VHX0 · AlphaFold monomer V2.0 prediction for voltage-dependent calcium channel gamma-3 subunit… · a predicted model of the same protein · chain D against A, TM-score 0.30

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P19491
• Every structure of UniProt Q8VHX0
• Elsewhere: RCSB PDB 7TNP · PDBe 7TNP · UniProt P19491 · UniProt Q8VHX0