MolNFT v1 · #186093 · protein + DNA + RNA
Cryo-EM structure of human telomerase-DNA-TPP1-POT1 complex (with POT1 side chains)
7QXS · Telomerase reverse transcriptase: “Cryo-EM structure of human telomerase-DNA-TPP1-POT1 complex (with POT1 side chains)”. Electron microscopy, 20 pockets (best druggability 0.85). MolNFT v1 #186093 on GenesisL1: 3D structure, binding…
- PDB
- 7QXS
- Type
- protein + DNA + RNA
- Length
- 1,772 residues
- Chains
- 7
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Telomerase reverse transcriptase; RNA (256-mer); Histone H2A; Histone H2B; Telomeric DNA…
- EC
- 2.7.7.49
- UniProt
- O14746, B2R5B3, B4DR52, A0A590TQL1, Q9NUX5
- Pockets
- 20 · best druggability 0.85
Structure
electron microscopy · 7 distinct chains · 42% helix, 19% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Telomerase reverse transcriptase (EC 2.7.7.49) | protein | 951 | 46% helix, 13% strand | O14746 |
| B | RNA (256-mer) | rna | 256 | — | — |
| L | Histone H2A | protein | 82 | 66% helix, 4% strand | B2R5B3 |
| M | Histone H2B | protein | 90 | 77% helix, 3% strand | B4DR52 |
| N | Telomeric DNA | dna | 22 | — | — |
| O | Adrenocortical dysplasia homolog (mouse), isoform CRA_A | protein | 120 | 23% helix, 38% strand | A0A590TQL1 |
| P | Protection of telomeres protein 1 | protein | 251 | 16% helix, 43% strand | Q9NUX5 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1117 | 0.85 | 0.86 | 135 | empty |
| 2 | 1024 | 0.83 | 0.84 | 61 | empty |
| 3 | 1004 | 0.81 | 0.82 | 84 | empty |
| 4 | 324 | 0.78 | 0.89 | 38 | empty |
| 5 | 1146 | 0.76 | 0.79 | 72 | empty |
Interfaces
10 interfaces between chains.
- A–B · 6435 Ų buried · 133 hydrogen bonds · protein-nucleic
- L–M · 2526 Ų buried · 15 hydrogen bonds · protein-protein
- A–N · 1743 Ų buried · 29 hydrogen bonds · protein-nucleic
- A–O · 1099 Ų buried · 16 hydrogen bonds · protein-protein
- N–P · 1080 Ų buried · 14 hydrogen bonds · protein-nucleic
Modifications
Observed in the structure: 1 disulfide (disulfide).
Compared structures
- 7QXS · Cryo-EM structure of human telomerase-DNA-TPP1-POT1 complex (with POT1 side chains) · the same entry in another collection · chain P against P, TM-score 1.00
- Q9NUX5 · AlphaFold monomer V2.0 prediction for protection of telomeres protein 1 (Q9NUX5) · a predicted model of the same protein · chain P against A, TM-score 0.99
- O14746 · AlphaFold monomer V2.0 prediction for telomerase reverse transcriptase (O14746) · a predicted model of the same protein · chain A against A, TM-score 0.91
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt O14746
- Every structure of UniProt B2R5B3
- Every structure of UniProt B4DR52
- Every structure of UniProt A0A590TQL1
- Every structure of UniProt Q9NUX5
- Elsewhere: RCSB PDB 7QXS · PDBe 7QXS · UniProt O14746 · UniProt B2R5B3 · UniProt B4DR52 · UniProt A0A590TQL1 · UniProt Q9NUX5
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