Full length ALPHA1 glycine receptor in presence of 1MM glycine and 32UM tetrahydrocannabinol state 1

Structure

electron microscopy · 1 distinct chain · 37% helix, 25% strand (DSSP).

Ligands and binding sites

• GLY · Glycine · ligand · 5 copies · chains A, B, E, C, D
• TCI · (6aR,10aR)-6,6,9-trimethyl-3-pentyl-6a,7,8,10a-tetrahydro-6H-benzo[c]chromen-1-ol · ligand · 5 copies · chains A, B, C, D, E
• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 5 copies · chains A, B, C, D, E

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

20 pockets found on a 1 Å grid, 18 with a druggability of 0.5 or more.

Interfaces

5 interfaces between chains.

• A–B · 2348 Ų buried · 14 hydrogen bonds · protein-protein
• C–D · 2293 Ų buried · 13 hydrogen bonds · protein-protein
• A–E · 2281 Ų buried · 13 hydrogen bonds · protein-protein
• B–C · 2249 Ų buried · 13 hydrogen bonds · protein-protein
• D–E · 2243 Ų buried · 10 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 5 disulfide, 5 glycan (disulfide, N-glycosylation).

Compared structures

• 7M6Q · Full length alpha1 Glycine receptor in presence of 1mM Glycine and 32uM… · the same entry in another collection · chain E against E, TM-score 1.00
• O93430 · AlphaFold monomer V2.0 prediction for glycine receptor subunit ALPHAZ1 (O93430) · a predicted model of the same protein · chain B against A, TM-score 0.96

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt O93430
• Elsewhere: RCSB PDB 7M6Q · PDBe 7M6Q · UniProt O93430