MolNFT v1 · #181436 · protein
Cryo-EM structure of the yeast tho-SUB2 complex
7LUV · Tho complex subunit HPR1: “Cryo-EM structure of the yeast tho-SUB2 complex”. Electron microscopy, 20 pockets (best druggability 0.84). MolNFT v1 #181436 on GenesisL1: 3D structure, binding sites and pockets in GL1X.
- PDB
- 7LUV
- Type
- protein
- Length
- 2,378 residues
- Chains
- 6
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Tho complex subunit HPR1; Tho complex subunit THP2; Tho complex subunit 2; Tho complex…
- EC
- 3.6.4.13
- UniProt
- P17629, O13539, P53552, P33441, Q07478
- Pockets
- 20 · best druggability 0.84
Structure
electron microscopy · 6 distinct chains · 55% helix, 8% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Tho complex subunit HPR1 | protein | 483 | 60% helix, 0% strand | P17629 |
| B | Tho complex subunit THP2 | protein | 189 | 73% helix, 1% strand | O13539 |
| C | Tho complex subunit 2 | protein | 911 | 65% helix, 2% strand | P53552 |
| D | Tho complex subunit MFT1 | protein | 150 | 85% helix, 0% strand | P33441 |
| E | TEX1 | protein | 271 | 2% helix, 45% strand | — |
| M | ATP-dependent RNA helicase SUB2 (EC 3.6.4.13) | protein | 374 | 40% helix, 15% strand | Q07478 |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1163 | 0.84 | 0.84 | 95 | empty |
| 2 | 977 | 0.83 | 0.84 | 80 | empty |
| 3 | 696 | 0.79 | 0.84 | 62 | empty |
| 4 | 126 | 0.77 | 0.95 | 25 | empty |
| 5 | 1111 | 0.76 | 0.78 | 80 | empty |
Interfaces
11 interfaces between chains.
- A–C · 5545 Ų buried · 26 hydrogen bonds · protein-protein
- B–D · 3972 Ų buried · 17 hydrogen bonds · protein-protein
- A–B · 2009 Ų buried · 7 hydrogen bonds · protein-protein
- C–E · 1792 Ų buried · 14 hydrogen bonds · protein-protein
- A–D · 1773 Ų buried · 5 hydrogen bonds · protein-protein
Compared structures
- 7LUV · Cryo-EM structure of the yeast THO-Sub2 complex · the same entry in another collection · chain M against M, TM-score 1.00
- P53552 · AlphaFold monomer V2.0 prediction for tho complex subunit 2 (P53552) · a predicted model of the same protein · chain C against A, TM-score 0.88
- Q07478 · AlphaFold monomer V2.0 prediction for ATP-dependent RNA helicase SUB2 (Q07478) · a predicted model of the same protein · chain M against A, TM-score 0.65
- P33441 · AlphaFold monomer V2.0 prediction for tho complex subunit MFT1 (P33441) · a predicted model of the same protein · chain D against A, TM-score 0.51
- P17629 · AlphaFold monomer V2.0 prediction for tho complex subunit HPR1 (P17629) · a predicted model of the same protein · chain A against A, TM-score 0.48
- O13539 · AlphaFold monomer V2.0 prediction for tho complex subunit THP2 (O13539) · a predicted model of the same protein · chain B against A, TM-score 0.38
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P17629
- Every structure of UniProt O13539
- Every structure of UniProt P53552
- Every structure of UniProt P33441
- Every structure of UniProt Q07478
- Elsewhere: RCSB PDB 7LUV · PDBe 7LUV · UniProt P17629 · UniProt O13539 · UniProt P53552 · UniProt P33441 · UniProt Q07478
GL1X is a live explorer of the GenesisL1 blockchain. Turn on JavaScript for search, charts and interactive views.