MolNFT v1 · #179360 · protein
Cryo-EM structure of stripak complex
7K36 · SERINE/THREONINE-protein phosphatase 2A 65 kda regulatory…: “Cryo-EM structure of stripak complex”. Electron microscopy, 3 ligands (IHP, MN, ZN), 20 pockets (best druggability 0.83). MolNFT v1 #179360 on GenesisL1: 3D structure…
- PDB
- 7K36
- Type
- protein
- Length
- 2,183 residues
- Chains
- 9
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- SERINE/THREONINE-protein phosphatase 2A 65 kda regulatory subunit a alpha isoform…
- EC
- 3.1.3.16
- UniProt
- P30153, Q13033, P67775, Q9Y3A3, Q5VSL9
- Ligands
- IHP, MN, ZN
- Pockets
- 20 · best druggability 0.83
Structure
electron microscopy · 9 distinct chains · 59% helix, 8% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | SERINE/THREONINE-protein phosphatase 2A 65 kda regulatory subunit a alpha isoform | protein | 554 | 79% helix, 0% strand | P30153 |
| B | Striatin-3 | protein | 298 | 1% helix, 44% strand | Q13033 |
| C | SERINE/THREONINE-protein phosphatase 2A catalytic subunit alpha isoform (EC 3.1.3.16) | protein | 293 | 35% helix, 17% strand | P67775 |
| D | Striatin-3 | protein | 74 | 95% helix, 0% strand | Q13033 |
| E | Striatin-3 | protein | 66 | 94% helix, 0% strand | Q13033 |
| F | Striatin-3 | protein | 62 | 94% helix, 0% strand | Q13033 |
| G | Striatin-3 | protein | 60 | 93% helix, 0% strand | Q13033 |
| H | Mob-like protein phocein | protein | 177 | 59% helix, 0% strand | Q9Y3A3 |
| I | Striatin-interacting protein 1 | protein | 599 | 65% helix, 1% strand | Q5VSL9 |
Ligands and binding sites
- IHP · Inositol hexakisphosphate · ligand · chains I
- MN · Manganese (II) ion · metal · 2 copies · chains C
- ZN · Zinc ion · metal · 2 copies · chains H
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
20 pockets found on a 1 Å grid, 14 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1179 | 0.83 | 0.83 | 97 | IHP |
| 2 | 1066 | 0.79 | 0.81 | 73 | empty |
| 3 | 889 | 0.79 | 0.82 | 92 | empty |
| 4 | 922 | 0.78 | 0.81 | 92 | empty |
| 5 | 108 | 0.73 | 0.93 | 23 | empty |
Interfaces
19 interfaces between chains.
- B–H · 1777 Ų buried · 23 hydrogen bonds · protein-protein
- D–E · 1500 Ų buried · 5 hydrogen bonds · protein-protein
- C–I · 1311 Ų buried · 10 hydrogen bonds · protein-protein
- E–I · 1051 Ų buried · 9 hydrogen bonds · protein-protein
- A–C · 986 Ų buried · 17 hydrogen bonds · protein-protein
Compared structures
- 7K36 · Cryo-EM structure of STRIPAK complex · the same entry in another collection · chain I against I, TM-score 1.00
- P67775 · AlphaFold monomer V2.0 prediction for SERINE/THREONINE-protein phosphatase 2A catalytic… · a predicted model of the same protein · chain C against A, TM-score 0.99
- Q13033 · AlphaFold monomer V2.0 prediction for striatin-3 (Q13033) · a predicted model of the same protein · chain B against A, TM-score 0.98
- Q5VSL9 · AlphaFold monomer V2.0 prediction for striatin-interacting protein 1 (Q5VSL9) · a predicted model of the same protein · chain I against A, TM-score 0.96
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P30153
- Every structure of UniProt Q13033
- Every structure of UniProt P67775
- Every structure of UniProt Q9Y3A3
- Every structure of UniProt Q5VSL9
- Elsewhere: RCSB PDB 7K36 · PDBe 7K36 · UniProt P30153 · UniProt Q13033 · UniProt P67775 · UniProt Q9Y3A3 · UniProt Q5VSL9
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