Cryo-EM structure of the gastric proton pump K791S/E820D/Y340N/E936V/Y799W mutant in K+-occluded (K+)E2-alf state

Structure

electron microscopy · 2 distinct chains · 43% helix, 17% strand (DSSP).

Ligands and binding sites

• PCW · 1,2-dioleoyl-sn-glycero-3-phosphocholine · ligand · 3 copies · chains A, B
• ALF · Tetrafluoroaluminate ion · ligand · chains A
• CLR · Cholesterol · ligand · chains A, B
• NAG · 2-acetamido-2-deoxy-beta-D-glucopyranose · sugar · 5 copies · chains B
• K · Potassium ion · metal · 3 copies · chains A
• MG · Magnesium ion · metal · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

15 pockets found on a 1 Å grid, 6 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 2255 Ų buried · 37 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 5 glycan, 3 disulfide (N-glycosylation, disulfide).

Compared structures

• 7ET1 · Cryo-EM structure of the gastric proton pump K791S/E820D/Y340N/E936V/Y799W mutant in… · the same entry in another collection · chain A against A, TM-score 1.00
• P18434 · AlphaFold monomer V2.0 prediction for potassium-transporting ATPase subunit beta (P18434) · a predicted model of the same protein · chain B against A, TM-score 0.96

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt A0A5G2QYH2
• Every structure of UniProt P18434
• Elsewhere: RCSB PDB 7ET1 · PDBe 7ET1 · UniProt A0A5G2QYH2 · UniProt P18434