Crystal structure of wild-type KRAS (gmppnp-bound) in complex with RAS-binding domain (rbd) and cysteine-rich domain (crd) of RAF1/CRAF (crystal form I)

Structure

x-ray diffraction · 2 distinct chains · 30% helix, 32% strand (DSSP).

Ligands and binding sites

• GNP · Phosphoaminophosphonic acid-guanylate ester · ligand · chains A
• ZN · Zinc ion · metal · 3 copies · chains B
• MG · Magnesium ion · metal · chains A
• CL · Chloride ion · ion · 5 copies · chains A, B
• SO4 · Sulfate ion · ion · 2 copies · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

1 pocket found on a 1 Å grid, 1 with a druggability of 0.5 or more.

Interfaces

1 interface between chains.

• A–B · 1286 Ų buried · 30 hydrogen bonds · protein-protein

Compared structures

• 6XI7 · Crystal Structure of wild-type KRAS (GMPPNP-bound) in complex with RAS-binding domain… · the same entry in another collection · chain A against A, TM-score 1.00
• P04049 · AlphaFold monomer V2.0 prediction for RAF proto-oncogene SERINE/THREONINE-protein kinase… · a predicted model of the same protein · chain B against A, TM-score 0.77

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt P04049
• Elsewhere: RCSB PDB 6XI7 · PDBe 6XI7 · UniProt P04049