MolNFT v1 · #143594 · protein + RNA
Cryo-EM structure of type I-F CRISPR crrna-guided csy surveillance complex with bound anti-CRISPR protein ACRF10
6B48 · CRISPR-associated protein CSY1: “Cryo-EM structure of type I-F CRISPR crrna-guided csy surveillance complex with bound anti-CRISPR protein…”. Electron microscopy, 20 pockets (best druggability 0.88). MolNFT v1 #143594 on GenesisL1…
- PDB
- 6B48
- Type
- protein + RNA
- Length
- 3,036 residues
- Chains
- 11
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR-associated protein CSY1; CRISPR-associated protein CSY2; CRISPR-associated protein…
- EC
- 3.1.-.-
- UniProt
- Q02ML9, Q02MM0, Q02MM1, A0A073KP86, Q02MM2
- Pockets
- 20 · best druggability 0.88
Structure
electron microscopy · 9 distinct chains · 25% helix, 15% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | CRISPR-associated protein CSY1 | protein | 424 | 38% helix, 4% strand | Q02ML9 |
| B | CRISPR-associated protein CSY2 | protein | 305 | 18% helix, 18% strand | Q02MM0 |
| C | CRISPR-associated protein CSY3 | protein | 293 | 23% helix, 16% strand | Q02MM1 |
| D, G, H | CRISPR-associated protein CSY3 | protein | 333 | 24% helix, 18% strand | Q02MM1 |
| E | CRISPR-associated protein CSY3 | protein | 334 | 24% helix, 22% strand | Q02MM1 |
| F | CRISPR-associated protein CSY3 | protein | 335 | 22% helix, 22% strand | Q02MM1 |
| K | Anti-CRISPR protein ACRF10 | protein | 97 | 14% helix, 23% strand | A0A073KP86 |
| L | CRISPR-associated endonuclease CAS6/CSY4 (EC 3.1.-.-) | protein | 189 | 21% helix, 2% strand | Q02MM2 |
| M | Pseudomonas aeruginosa strain SMC4485 CRISPR repeat sequence | rna | 60 | — | — |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 965 | 0.88 | 0.90 | 96 | empty |
| 2 | 183 | 0.82 | 0.97 | 48 | empty |
| 3 | 810 | 0.80 | 0.83 | 88 | empty |
| 4 | 852 | 0.79 | 0.82 | 80 | empty |
| 5 | 867 | 0.78 | 0.81 | 80 | empty |
Interfaces
27 interfaces between chains.
- A–B · 3558 Ų buried · 33 hydrogen bonds · protein-protein
- F–G · 2221 Ų buried · 23 hydrogen bonds · protein-protein
- D–E · 2202 Ų buried · 26 hydrogen bonds · protein-protein
- G–H · 2189 Ų buried · 35 hydrogen bonds · protein-protein
- E–F · 2071 Ų buried · 24 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 5 modified (engineered).
Compared structures
- 6B48 · Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound… · the same entry in another collection · chain M against M, TM-score 1.00
- Q02MM1 · AlphaFold monomer V2.0 prediction for CRISPR-associated protein CSY3 (Q02MM1) · a predicted model of the same protein · chain G against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q02ML9
- Every structure of UniProt Q02MM0
- Every structure of UniProt Q02MM1
- Every structure of UniProt A0A073KP86
- Every structure of UniProt Q02MM2
- Elsewhere: RCSB PDB 6B48 · PDBe 6B48 · UniProt Q02ML9 · UniProt Q02MM0 · UniProt Q02MM1 · UniProt A0A073KP86 · UniProt Q02MM2
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