MolNFT v1 · #143593 · protein + RNA

Cryo-EM structure of type I-F CRISPR crrna-guided csy surveillance complex with bound anti-CRISPR protein ACRF2

6B47 · CRISPR-associated protein CSY1: “Cryo-EM structure of type I-F CRISPR crrna-guided csy surveillance complex with bound anti-CRISPR protein…”. Electron microscopy, 20 pockets (best druggability 0.83). MolNFT v1 #143593 on GenesisL1…

PDB: 6B47
Type: protein + RNA
Length: 3,025 residues
Chains: 11
Method: electron microscopy
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: CRISPR-associated protein CSY1; CRISPR-associated protein CSY2; CRISPR-associated protein…
EC: 3.1.-.-
UniProt: Q02ML9, Q02MM0, Q02MM1, Q6TM72, Q02MM2
Pockets: 20 · best druggability 0.83

Structure

electron microscopy · 8 distinct chains · 28% helix, 20% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A	CRISPR-associated protein CSY1	protein	424	42% helix, 6% strand	Q02ML9
B	CRISPR-associated protein CSY2	protein	305	19% helix, 30% strand	Q02MM0
C	CRISPR-associated protein CSY3	protein	293	26% helix, 25% strand	Q02MM1
D, G, H	CRISPR-associated protein CSY3	protein	333	25% helix, 23% strand	Q02MM1
E, F	CRISPR-associated protein CSY3	protein	334	27% helix, 25% strand	Q02MM1
K	Anti-CRISPR protein ACRF2	protein	87	31% helix, 25% strand	Q6TM72
L	CRISPR-associated endonuclease CAS6/CSY4 (EC 3.1.-.-)	protein	189	20% helix, 6% strand	Q02MM2
M	Pseudomonas aeruginosa strain SMC4485 CRISPR repeat sequence	rna	60	—	—

Pockets

20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	915	0.83	0.85	115	empty
2	891	0.81	0.83	102	empty
3	1029	0.80	0.82	106	empty
4	1022	0.79	0.81	97	empty
5	1128	0.78	0.80	118	empty

Interfaces

26 interfaces between chains.

A–B · 3677 Å² buried · 34 hydrogen bonds · protein-protein
G–H · 2086 Å² buried · 25 hydrogen bonds · protein-protein
D–E · 2060 Å² buried · 30 hydrogen bonds · protein-protein
C–D · 2057 Å² buried · 28 hydrogen bonds · protein-protein
E–F · 1975 Å² buried · 34 hydrogen bonds · protein-protein

Compared structures

6B47 · Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound… · the same entry in another collection · chain M against M, TM-score 1.00
Q02MM1 · AlphaFold monomer V2.0 prediction for CRISPR-associated protein CSY3 (Q02MM1) · a predicted model of the same protein · chain C against A, TM-score 0.98

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt Q02ML9
Every structure of UniProt Q02MM0
Every structure of UniProt Q02MM1
Every structure of UniProt Q6TM72
Every structure of UniProt Q02MM2
Elsewhere: RCSB PDB 6B47 · PDBe 6B47 · UniProt Q02ML9 · UniProt Q02MM0 · UniProt Q02MM1 · UniProt Q6TM72 · UniProt Q02MM2

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