MolNFT v1 · #143591 · protein + RNA
Cryo-EM structure of type I-F CRISPR crrna-guided csy surveillance complex
6B45 · CRISPR-associated protein CSY1: “Cryo-EM structure of type I-F CRISPR crrna-guided csy surveillance complex”. Electron microscopy, 20 pockets (best druggability 0.83). MolNFT v1 #143591 on GenesisL1: 3D structure, binding sites and…
- PDB
- 6B45
- Type
- protein + RNA
- Length
- 2,939 residues
- Chains
- 10
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR-associated protein CSY1; CRISPR-associated protein CSY2; CRISPR-associated protein…
- EC
- 3.1.-.-
- UniProt
- Q02ML9, Q02MM0, Q02MM1, Q02MM2
- Pockets
- 20 · best druggability 0.83
Structure
electron microscopy · 8 distinct chains · 23% helix, 15% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | CRISPR-associated protein CSY1 | protein | 424 | 33% helix, 5% strand | Q02ML9 |
| B | CRISPR-associated protein CSY2 | protein | 305 | 21% helix, 16% strand | Q02MM0 |
| C | CRISPR-associated protein CSY3 | protein | 293 | 22% helix, 13% strand | Q02MM1 |
| D, G, H | CRISPR-associated protein CSY3 | protein | 333 | 19% helix, 21% strand | Q02MM1 |
| E | CRISPR-associated protein CSY3 | protein | 334 | 21% helix, 18% strand | Q02MM1 |
| F | CRISPR-associated protein CSY3 | protein | 335 | 21% helix, 21% strand | Q02MM1 |
| L | CRISPR-associated endonuclease CAS6/CSY4 (EC 3.1.-.-) | protein | 189 | 16% helix, 6% strand | Q02MM2 |
| M | Pseudomonas aeruginosa strain SMC4485 CRISPR repeat sequence | rna | 60 | — | — |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 929 | 0.83 | 0.85 | 91 | empty |
| 2 | 901 | 0.80 | 0.83 | 98 | empty |
| 3 | 898 | 0.79 | 0.82 | 94 | empty |
| 4 | 1009 | 0.79 | 0.81 | 104 | empty |
| 5 | 1041 | 0.79 | 0.81 | 95 | empty |
Interfaces
24 interfaces between chains.
- A–B · 3317 Ų buried · 32 hydrogen bonds · protein-protein
- F–G · 2127 Ų buried · 26 hydrogen bonds · protein-protein
- E–F · 2053 Ų buried · 27 hydrogen bonds · protein-protein
- D–E · 2049 Ų buried · 26 hydrogen bonds · protein-protein
- G–H · 2027 Ų buried · 28 hydrogen bonds · protein-protein
Compared structures
- 6B45 · Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex · the same entry in another collection · chain M against M, TM-score 1.00
- Q02MM1 · AlphaFold monomer V2.0 prediction for CRISPR-associated protein CSY3 (Q02MM1) · a predicted model of the same protein · chain G against A, TM-score 0.97
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q02ML9
- Every structure of UniProt Q02MM0
- Every structure of UniProt Q02MM1
- Every structure of UniProt Q02MM2
- Elsewhere: RCSB PDB 6B45 · PDBe 6B45 · UniProt Q02ML9 · UniProt Q02MM0 · UniProt Q02MM1 · UniProt Q02MM2
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