MolNFT v1 · #136633 · protein + RNA
Cryo EM structure of anti-crisprs, ACRF1 and ACRF2, bound to type I-F crrna-guided CRISPR surveillance complex
5UZ9 · CRISPR-associated protein CSY1: “Cryo EM structure of anti-crisprs, ACRF1 and ACRF2, bound to type I-F crrna-guided CRISPR surveillance complex”. Electron microscopy, 20 pockets (best druggability 0.85). MolNFT v1 #136633 on…
- PDB
- 5UZ9
- Type
- protein + RNA
- Length
- 3,179 residues
- Chains
- 13
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- CRISPR-associated protein CSY1; CRISPR-associated protein CSY2; CRISPR-associated protein…
- EC
- 3.1.-.-
- UniProt
- Q02ML9, Q02MM0, Q02MM1, L7P7M1, Q6TM72, Q02MM2
- Pockets
- 20 · best druggability 0.85
Structure
electron microscopy · 10 distinct chains · 27% helix, 20% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | CRISPR-associated protein CSY1 | protein | 424 | 43% helix, 6% strand | Q02ML9 |
| B | CRISPR-associated protein CSY2 | protein | 305 | 20% helix, 24% strand | Q02MM0 |
| C | CRISPR-associated protein CSY3 | protein | 293 | 25% helix, 23% strand | Q02MM1 |
| D, G, H | CRISPR-associated protein CSY3 | protein | 333 | 24% helix, 23% strand | Q02MM1 |
| E | CRISPR-associated protein CSY3 | protein | 334 | 24% helix, 22% strand | Q02MM1 |
| F | CRISPR-associated protein CSY3 | protein | 335 | 24% helix, 23% strand | Q02MM1 |
| I, J | Anti-CRISPR protein ACR30-35 | protein | 76 | 25% helix, 37% strand | L7P7M1 |
| K | Anti-CRISPR protein 30 | protein | 88 | 27% helix, 23% strand | Q6TM72 |
| L | CRISPR-associated endonuclease CAS6/CSY4 (EC 3.1.-.-) | protein | 189 | 23% helix, 13% strand | Q02MM2 |
| M | CRISPR RNA (60-mer) | rna | 60 | — | — |
Pockets
20 pockets found on a 1 Å grid, 20 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1056 | 0.85 | 0.86 | 133 | empty |
| 2 | 798 | 0.84 | 0.87 | 81 | empty |
| 3 | 1062 | 0.83 | 0.84 | 100 | empty |
| 4 | 1141 | 0.83 | 0.84 | 122 | empty |
| 5 | 1054 | 0.83 | 0.84 | 115 | empty |
Interfaces
34 interfaces between chains.
- A–B · 4438 Ų buried · 53 hydrogen bonds · protein-protein
- F–G · 2060 Ų buried · 22 hydrogen bonds · protein-protein
- C–D · 1989 Ų buried · 23 hydrogen bonds · protein-protein
- D–E · 1935 Ų buried · 19 hydrogen bonds · protein-protein
- E–F · 1893 Ų buried · 19 hydrogen bonds · protein-protein
Compared structures
- 5UZ9 · Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR… · the same entry in another collection · chain M against M, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q02ML9
- Every structure of UniProt Q02MM0
- Every structure of UniProt Q02MM1
- Every structure of UniProt L7P7M1
- Every structure of UniProt Q6TM72
- Every structure of UniProt Q02MM2
- Elsewhere: RCSB PDB 5UZ9 · PDBe 5UZ9 · UniProt Q02ML9 · UniProt Q02MM0 · UniProt Q02MM1 · UniProt L7P7M1 · UniProt Q6TM72 · UniProt Q02MM2
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