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MolNFT v1 · #12869 · protein + DNA

Crystal structure of thermus aquatics RNA polymerase sigmaa subunit region 4 bound to-35 element DNA

1KU7 · 5'-D(*CP*CP*TP*TP*GP*AP*CP*AP*AP*AP*G)-3': “Crystal structure of thermus aquatics RNA polymerase sigmaa subunit region 4 bound to-35 element DNA”. X-ray diffraction. MolNFT v1 #12869 on GenesisL1: 3D structure, binding sites and…

PDB
1KU7
Type
protein + DNA
Length
163 residues
Chains
4
Method
x-ray diffraction
Collection
MolNFT v1 / PDB
Contract
0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner
0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule
5'-D(*CP*CP*TP*TP*GP*AP*CP*AP*AP*AP*G)-3'; 5'-D(*CP*CP*TP*TP*TP*GP*TP*CP*AP*AP*G)-3'…
UniProt
Q9EZJ8

Structure

x-ray diffraction · 4 distinct chains · 65% helix, 0% strand (DSSP).

ChainMoleculeTypeResiduesSecondary structureUniProt
B5'-D(*CP*CP*TP*TP*GP*AP*CP*AP*AP*AP*G)-3'dna11——
C5'-D(*CP*CP*TP*TP*TP*GP*TP*CP*AP*AP*G)-3'dna11——
ASigma factor sigaprotein7367% helix, 0% strandQ9EZJ8
DSigma factor sigaprotein6863% helix, 0% strandQ9EZJ8

Interfaces

4 interfaces between chains.

  • A–D · 1004 Ų buried · 5 hydrogen bonds · protein-protein
  • B–C · 444 Ų buried · 30 hydrogen bonds · nucleic-nucleic
  • C–A · 369 Ų buried · 7 hydrogen bonds · protein-nucleic
  • B–A · 313 Ų buried · 7 hydrogen bonds · protein-nucleic

Compared structures

  • 1KU7 · Crystal Structure of Thermus aquatics RNA Polymerase SigmaA Subunit Region 4 Bound to-35… · the same entry in another collection · chain B against B, TM-score 1.00
  • Q9EZJ8 · AlphaFold monomer V2.0 prediction for RNA polymerase sigma factor siga (Q9EZJ8) · a predicted model of the same protein · chain A against A, TM-score 0.80

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