Human DNA polymerase eta-DNA ternary complex: misincorporation G opposite T after an a at the primer 3' end (AA/G)

Structure

x-ray diffraction · 3 distinct chains · 45% helix, 23% strand (DSSP).

Ligands and binding sites

• XG4 · 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine · ligand · chains A, T, P
• MG · Magnesium ion · metal · 2 copies · chains A
• NA · Sodium ion · metal · chains A
• GOL · Glycerol · additive · 2 copies · chains A

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

6 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.

Interfaces

3 interfaces between chains.

• A–T · 969 Ų buried · 24 hydrogen bonds · protein-nucleic
• A–P · 617 Ų buried · 14 hydrogen bonds · protein-nucleic
• T–P · 380 Ų buried · 35 hydrogen bonds · nucleic-nucleic

Compared structures

• 4J9M · Human DNA polymerase eta-DNA ternary complex: misincorporation G opposite T after an A at… · the same entry in another collection · chain A against A, TM-score 1.00

Analyse

• Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
• Every structure of UniProt Q9Y253
• Elsewhere: RCSB PDB 4J9M · PDBe 4J9M · UniProt Q9Y253