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Crystal structure of the E. COLI beta clamp mutant R103C, I305C, C260S, C333S at 2.2A resolution

Analyse Crystal structure of the E. COLI beta clamp mutant R103C, I305C, C260S, C333S… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 3PWE
Type: protein
Length: 732 residues
Chains: 2
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: DNA polymerase III subunit beta
EC: 2.7.7.7
UniProt: P0A988
Pockets: 5 · best druggability 0.44

Structure

x-ray diffraction · 1 distinct chain · 22% helix, 45% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A, B	DNA polymerase III subunit beta (EC 2.7.7.7)	protein	366	22% helix, 45% strand	P0A988

Pockets

5 pockets found on a 1 Å grid, 0 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	187	0.44	0.74	29	empty
2	180	0.43	0.74	26	empty
3	58	0.30	0.75	17	empty
4	66	0.30	0.74	16	empty
5	51	0.25	0.73	19	empty

Interfaces

1 interface between chains.

A–B · 1318 Å² buried · 28 hydrogen bonds · protein-protein

Compared structures

3PWE · Crystal structure of the E. coli beta clamp mutant R103C, I305C, C260S, C333S at 2.2A… · the same entry in another collection · chain A against A, TM-score 1.00
P0A988 · AlphaFold monomer V2.0 prediction for beta sliding clamp (P0A988) · a predicted model of the same protein · chain B against A, TM-score 0.98

Analyse

Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P0A988
Elsewhere: RCSB PDB 3PWE · PDBe 3PWE · UniProt P0A988

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