Research workbench
Visualizing ATP-dependent RNA translocation by the NS3 helicase from HCV
Analyse Visualizing ATP-dependent RNA translocation by the NS3 helicase from HCV in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3O8D
- Type
- protein
- Length
- 1,290 residues
- Chains
- 2
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- HCV NS3 PROTEASE/HELICASE
- EC
- 3.4.21.98, 3.6.1.15, 3.6.4.13
- UniProt
- Q99AU2
- Ligands
- ADP, BEF, MG, SO4
- Pockets
- 18 · best druggability 0.80
Structure
x-ray diffraction · 1 distinct chain · 28% helix, 29% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, B | HCV NS3 PROTEASE/HELICASE (EC 3.4.21.98, 3.6.1.15, 3.6.4.13) | protein | 645 | 28% helix, 29% strand | Q99AU2 |
Ligands and binding sites
- ADP · Adenosine-5'-diphosphate · ligand · 2 copies · chains A, B
- BEF · Beryllium trifluoride ion · ligand · 2 copies · chains A, B
- MG · Magnesium ion · metal · 2 copies · chains A, B
- SO4 · Sulfate ion · ion · 2 copies · chains A, B
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
18 pockets found on a 1 Å grid, 11 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 1033 | 0.80 | 0.81 | 105 | SO4 |
| 2 | 970 | 0.79 | 0.81 | 105 | SO4 |
| 3 | 487 | 0.67 | 0.78 | 45 | empty |
| 4 | 398 | 0.63 | 0.77 | 48 | empty |
| 5 | 451 | 0.60 | 0.75 | 41 | empty |
Interfaces
1 interface between chains.
- A–B · 1406 Ų buried · 10 hydrogen bonds · protein-protein
Modifications
Observed in the structure: 1 disulfide (disulfide).
Compared structures
- 3O8D · Visualizing ATP-dependent RNA Translocation by the NS3 Helicase from HCV · the same entry in another collection · chain A against A, TM-score 1.00
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt Q99AU2
- Elsewhere: RCSB PDB 3O8D · PDBe 3O8D · UniProt Q99AU2
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