Research workbench
EM structure of the heavy meromyosin subfragment of chick smooth muscle myosin with regulatory light chain in phosphorylated state
Analyse EM structure of the heavy meromyosin subfragment of chick smooth muscle myosin… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3J04
- Type
- protein
- Length
- 2,388 residues
- Chains
- 6
- Method
- electron microscopy
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Myosin-11; Myosin regulatory light chain 2, smooth muscle major isoform; Myosin light…
- UniProt
- P10587, P02612, P02607
- Pockets
- 20 · best druggability 0.78
Structure
electron microscopy · 3 distinct chains · 47% helix, 10% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A, D | Myosin-11 | protein | 903 | 46% helix, 12% strand | P10587 |
| B, E | Myosin regulatory light chain 2, smooth muscle major isoform | protein | 143 | 47% helix, 0% strand | P02612 |
| C, F | Myosin light polypeptide 6 | protein | 148 | 52% helix, 7% strand | P02607 |
Pockets
20 pockets found on a 1 Å grid, 16 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 879 | 0.78 | 0.81 | 82 | empty |
| 2 | 898 | 0.77 | 0.80 | 80 | empty |
| 3 | 1058 | 0.76 | 0.78 | 88 | empty |
| 4 | 965 | 0.73 | 0.76 | 78 | empty |
| 5 | 87 | 0.72 | 0.95 | 21 | empty |
Interfaces
8 interfaces between chains.
- A–B · 1965 Ų buried · 23 hydrogen bonds · protein-protein
- D–E · 1877 Ų buried · 25 hydrogen bonds · protein-protein
- D–F · 1660 Ų buried · 24 hydrogen bonds · protein-protein
- A–C · 1432 Ų buried · 10 hydrogen bonds · protein-protein
- A–D · 946 Ų buried · 5 hydrogen bonds · protein-protein
Compared structures
- 3J04 · EM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with… · the same entry in another collection · chain F against F, TM-score 1.00
- P02607 · AlphaFold monomer V2.0 prediction for myosin light polypeptide 6 (P02607) · a predicted model of the same protein · chain C against A, TM-score 0.98
- P10587 · AlphaFold monomer V2.0 prediction for myosin-11 (P10587) · a predicted model of the same protein · chain A against A, TM-score 0.88
- P02612 · AlphaFold monomer V2.0 prediction for myosin regulatory light chain 2, smooth muscle… · a predicted model of the same protein · chain B against A, TM-score 0.80
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P10587
- Every structure of UniProt P02612
- Every structure of UniProt P02607
- Elsewhere: RCSB PDB 3J04 · PDBe 3J04 · UniProt P10587 · UniProt P02612 · UniProt P02607
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