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Crystal structure of the lasa virulence factor from pseudomonas aeruginosa

Analyse Crystal structure of the lasa virulence factor from pseudomonas aeruginosa in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.

PDB: 3IT7
Type: protein
Length: 364 residues
Chains: 2
Method: x-ray diffraction
Collection: MolNFT v1 / PDB
Contract: 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
Owner: 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
Molecule: Protease lasa
EC: 3.4.24.-
UniProt: P14789
Ligands: ZN
Pockets: 3 · best druggability 0.39

Structure

x-ray diffraction · 1 distinct chain · 4% helix, 36% strand (DSSP).

Chain	Molecule	Type	Residues	Secondary structure	UniProt
A, B	Protease lasa (EC 3.4.24.-)	protein	182	4% helix, 36% strand	P14789

Ligands and binding sites

ZN · Zinc ion · metal · 2 copies · chains A, B
GOL · Glycerol · additive · 3 copies · chains A, B
TLA · L(+)-tartaric acid · additive · 2 copies · chains A, B

Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.

Pockets

3 pockets found on a 1 Å grid, 0 with a druggability of 0.5 or more.

Pocket	Volume (Å³)	Druggability	Buriedness	Lining residues	Inside
1	142	0.39	0.73	24	empty
2	53	0.28	0.74	12	empty
3	55	0.25	0.73	18	TLA

Interfaces

1 interface between chains.

A–B · 160 Å² buried · 0 hydrogen bonds · protein-protein

Modifications

Observed in the structure: 4 disulfide (disulfide).

Compared structures

3IT7 · Crystal Structure of the LasA virulence factor from Pseudomonas aeruginosa · the same entry in another collection · chain A against A, TM-score 1.00
P14789 · AlphaFold monomer V2.0 prediction for protease lasa (P14789) · a predicted model of the same protein · chain A against A, TM-score 0.97

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Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
Every structure of UniProt P14789
Elsewhere: RCSB PDB 3IT7 · PDBe 3IT7 · UniProt P14789

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