Research workbench
Molecular architecture of galphao and the structural basis for RGS16- mediated deactivation
Analyse Molecular architecture of galphao and the structural basis for RGS16- mediated… in the GL1X research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides, crosslinks and neighbours on the 3D structure.
- PDB
- 3C7K
- Type
- protein
- Length
- 848 residues
- Chains
- 4
- Method
- x-ray diffraction
- Collection
- MolNFT v1 / PDB
- Contract
- 0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba
- Owner
- 0x3396CDB397284571d12822B4ae1189a8E22AAAAA
- Molecule
- Guanine nucleotide-binding protein G(O) subunit alpha; Regulator of G-protein signaling 16
- UniProt
- P18872, P97428
- Ligands
- ALF, GDP, MG
- Pockets
- 6 · best druggability 0.85
Structure
x-ray diffraction · 4 distinct chains · 52% helix, 7% strand (DSSP).
| Chain | Molecule | Type | Residues | Secondary structure | UniProt |
|---|---|---|---|---|---|
| A | Guanine nucleotide-binding protein G(O) subunit alpha | protein | 305 | 46% helix, 8% strand | P18872 |
| B | Regulator of G-protein signaling 16 | protein | 119 | 69% helix, 0% strand | P97428 |
| C | Guanine nucleotide-binding protein G(O) subunit alpha | protein | 311 | 48% helix, 11% strand | P18872 |
| D | Regulator of G-protein signaling 16 | protein | 113 | 61% helix, 0% strand | P97428 |
Ligands and binding sites
- ALF · Tetrafluoroaluminate ion · ligand · 2 copies · chains A, C
- GDP · Guanosine-5'-diphosphate · ligand · 2 copies · chains A, C
- MG · Magnesium ion · metal · 2 copies · chains A, C
Binding sites are the residues within 4.5 Å of a ligand's heavy atoms.
Pockets
6 pockets found on a 1 Å grid, 3 with a druggability of 0.5 or more.
| Volume (ų) | Druggability | Buriedness | Lining residues | Inside | |
|---|---|---|---|---|---|
| 1 | 957 | 0.85 | 0.86 | 87 | MG, ALF, GDP |
| 2 | 1190 | 0.82 | 0.83 | 101 | MG, ALF, GDP |
| 3 | 236 | 0.61 | 0.81 | 21 | empty |
| 4 | 131 | 0.48 | 0.78 | 15 | empty |
| 5 | 61 | 0.30 | 0.75 | 13 | empty |
Interfaces
2 interfaces between chains.
- A–B · 970 Ų buried · 20 hydrogen bonds · protein-protein
- C–D · 944 Ų buried · 13 hydrogen bonds · protein-protein
Compared structures
- 3C7K · Molecular architecture of Galphao and the structural basis for RGS16-mediated deactivation · the same entry in another collection · chain D against D, TM-score 1.00
- P18872 · AlphaFold monomer V2.0 prediction for guanine nucleotide-binding protein G(O) subunit… · a predicted model of the same protein · chain A against A, TM-score 0.99
- P97428 · AlphaFold monomer V2.0 prediction for regulator of G-protein signaling 16 (P97428) · a predicted model of the same protein · chain D against A, TM-score 0.98
Analyse
- Open in the research workbench: secondary structure, accessibility, pockets, interfaces, conservation, variants, modifications, peptides and crosslinks on the 3D structure
- Every structure of UniProt P18872
- Every structure of UniProt P97428
- Elsewhere: RCSB PDB 3C7K · PDBe 3C7K · UniProt P18872 · UniProt P97428
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